ANKRD11 Polyclonal antibody

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• KD/KO Validated

ANKRD11 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 55364-1-AP

Synonyms

ANCO 1, ANCO1, ANKRD11, ankyrin repeat domain 11, LZ16, T13

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Validation Data Gallery

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  WB analysis of HeLa using 55364-1-AP

  WB result of ANKRD11 antibody (55364-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-ANKRD11 transfected HeLa cells.

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  WB analysis of BxPC-3 using 55364-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 55364-1-AP (ANKRD11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55364-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55364-1-AP (ANKRD11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 55364-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 55364-1-AP (ANKRD11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55364-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55364-1-AP (ANKRD11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: BxPC-3 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

55364-1-AP targets ANKRD11 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: ankyrin repeat domain 11
• Calculated Molecular Weight: 298 kDa
• Observed Molecular Weight: 150-170 kDa
• GenBank Accession Number: NM_013275
• Gene Symbol: ANKRD11
• Gene ID (NCBI): 29123
• RRID: AB_11232213
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANKRD11, also named as ANCO1, may recruit HDACs to the p160 coactivators/nuclear receptor complex to inhibit ligand-dependent transactivation. This antibody is specific to ANKRD11. ANKRD11 exists 150-170 kDa isoform.

Publications

Species	Application	Title
human	WB	Am J Med Genet A; Two loss-of-function ANKRD11 variants in Chinese patients with short stature and a possible molecular pathway.; Authors - Tingting Zhang
mouse	IHC,IF	J Physiol Biochem; The effect of aspartame on accelerating caspase-dependent apoptosis of pancreatic islet via ZIPK/STAT3/caspase 3 signaling pathway; Authors - Haiying Hu