ANKRD26 Polyclonal antibody

ANKRD26 Polyclonal Antibody for IF, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 20035-1-AP

Synonyms

ANKRD26, ankyrin repeat domain 26, KIAA1074

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Validation Data Gallery

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  IP experiment of HeLa cells using 20035-1-AP

  IP result of anti-ANKRD26 (20035-1-AP for IP and Detection).

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  IHC staining of human breast cancer using 20035-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 20035-1-AP (ANKRD26 Antibody) at dilution of 1:50.

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  IHC staining of human colon using 20035-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 20035-1-AP (ANKRD26 Antibody) at dilution of 1:50.

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  IF Staining of HeLa using 20035-1-AP

  Immunofluorescent analysis of HeLa cells using 20035-1-AP (ANKRD26 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive IP detected in: HeLa cells
• Positive IHC detected in: human breast cancer tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

20035-1-AP targets ANKRD26 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: ankyrin repeat domain 26
• Calculated Molecular Weight: 196 kDa
• GenBank Accession Number: NM_014915
• Gene Symbol: ANKRD26
• Gene ID (NCBI): 22852
• RRID: AB_11183053
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANKRD26 also named as KIAA1074. The antibody recognizes the C-term of ANKRD26.

Publications

Species	Application	Title
mouse	WB	Cell; Transgenerational inheritance of acquired epigenetic signatures at CpG islands in mice; Authors - Yuta Takahashi