ANKRD31 Polyclonal antibody

ANKRD31 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Rat, mouse, human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29891-1-AP

Synonyms

ANKRD31, ankyrin repeat domain 31, FLJ40191

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Validation Data Gallery

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  WB analysis of rat testis using 29891-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 29891-1-AP (ANKRD31 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 29891-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29891-1-AP (ANKRD31 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: rat testis tissue, mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29891-1-AP targets ANKRD31 in WB, ELISA applications and shows reactivity with Rat, mouse, human samples.

• Tested Reactivity: Rat, mouse, human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ANKRD31 fusion protein Ag31858
• Full Name: ankyrin repeat domain 31
• Calculated Molecular Weight: 211 kDa
• Observed Molecular Weight: 210 kDa
• GenBank Accession Number: NM_001164443.1
• Gene Symbol: ANKRD31
• Gene ID (NCBI): 256006
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANKRD31 (Ankyrin repeat domain-containing protein 31) controls the number, timing, and location of meiotic DNA breaks during meiosis. It encodes a 1857 amino acid protein with 2 ankyrin repeat domains (PubMed: 31003867). ANKRD31 was a meiotic protein colocalized REC114 on chromatin (PubMed: 31000436). Mutations in ANKRD31 are associated with a Rett syndrome (RTT)-like phenotype (PMID: 27541642). And ANKRD31 has a high expression in testis.