Tested Applications
| Positive IHC detected in | human hepatocirrhosis tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24058-1-AP targets ANKS3 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21252 Product name: Recombinant human ANKS3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 179-527 aa of BC050387 Sequence: LEEEGLCCRDVTSPINERDVESSSSSSSREEHAFCANLGPVQSSSSSEGLARAQGLSSEASVESNEDSDHACKSSARKQAKSYMKTKNPDSQWPPRTATDREGFLAESSPQTQRAPYSGPQDLAALLEQIGCLKYLQVFEEQDVDLRIFLTLTESDLKEIGITLFGPKRKMTSAIARWHSSARPPGDALELAYADRLEAEMQELAIQLHKRCEEVEATRGQVCQEQELRAVVESCLLEQDRAREDLQARLRETWALARDAALVLDQLRACQAELSSRVRQDQPPGAATLGLAVPPADSKGWQASLQAMSLPELSGALEDRVREMGQALCLVTQSLEKLQVLSGKKWRET Predict reactive species |
| Full Name | ankyrin repeat and sterile alpha motif domain containing 3 |
| Calculated Molecular Weight | 656 aa, 72 kDa |
| Observed Molecular Weight | 72 kDa, 63 kDa |
| GenBank Accession Number | BC050387 |
| Gene Symbol | ANKS3 |
| Gene ID (NCBI) | 124401 |
| RRID | AB_2879418 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | Q6ZW76 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ANKS3 (ankyrin repeat and sterile alpha motif domain containing 3) is a 656 amino acid protein that contain six ANK repeats and one SAM (sterile alpha motif) domain. Each ANK repeat consists of a 30-34 amino acid sequence that exhibits a helix-turn-helix conformation and functions to mediate protein-protein interactions. The SAM domain is a 70 amino acid domain that also functions to mediate protein-protein interactions for homo- and hetero- oligomerization. Two isoforms of ANKS3 exist due to alternative splicing events.This antibody may recognize both of them.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ANKS3 antibody 24058-1-AP | Download protocol |
| IHC protocol for ANKS3 antibody 24058-1-AP | Download protocol |
| WB protocol for ANKS3 antibody 24058-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ANKS3 Polyclonal antibody (Cat# 24058-1-AP) has 1 total citation. It appears in PLoS Biology (2023), in a study on Bicc1 ribonucleoprotein complexes specifying organ laterality through ANKS6-induced structural remodeling of ANKS3. The research fields include developmental biology, organ laterality determination, and protein structural biology. The antibody was applied in WB, IP, IF, and CoIP in mouse and human samples.
| Species | Application | Title |
|---|---|---|
PLoS Biol Bicc1 ribonucleoprotein complexes specifying organ laterality are licensed by ANKS6-induced structural remodeling of associated ANKS3 |
