ANO10 Polyclonal antibody

ANO10 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 21901-1-AP

Synonyms

ANO10, anoctamin 10, TMEM16K, Transmembrane protein 16K

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Validation Data Gallery

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  WB analysis of mouse brain using 21901-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21901-1-AP (ANO10 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

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  WB analysis of mouse brain using 21901-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21901-1-AP (ANO10 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 21901-1-AP

  IP result of anti-ANO10 (IP:21901-1-AP, 3ug; Detection:21901-1-AP 1:500) with mouse brain tissue lysate 3600ug.

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  IHC staining of human testis using 21901-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 21901-1-AP (ANO10 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 21901-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 21901-1-AP (ANO10 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

The immunogen of 21901-1-AP is ANO10 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ANO10 fusion protein Ag13983
• Full Name: anoctamin 10
• Calculated Molecular Weight: 660 aa, 76 kDa
• Observed Molecular Weight: 76 kDa
• GenBank Accession Number: BC038855
• Gene Symbol: ANO10
• Gene ID (NCBI): 55129
• RRID: AB_2878940
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANO10, also named as TMEM16K, is 660 amino acid protein, which belongs to the anoctamin family. ANO10 does not exhibit calcium-activated chloride channel activity and can inhibit the activity of ANO1. ANO10 is Highly expressed in the brain. ANO10 may have an association with Spinocerebellar ataxia, autosomal recessive, 10.

Publications

Species	Application	Title
human	IHC	Am J Cancer Res; ANO10 is a potential prognostic biomarker and correlates with immune infiltration in breast cancer; Authors - Ran Ning