ANO2 Polyclonal antibody

• Featured Product
• KD/KO Validated

ANO2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 20647-1-AP

Synonyms

ANO2, anoctamin 2, C12orf3, TMEM16B, Transmembrane protein 16B

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Validation Data Gallery

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  WB analysis using 20647-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 20647-1-AP (ANO2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 20647-1-AP

  IP result of anti-ANO2 (IP:20647-1-AP, 4ug; Detection:20647-1-AP 1:300) with mouse brain tissue lysate 10000ug.

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  IHC staining of mouse eye using 20647-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 20647-1-AP (ANO2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse eye using 20647-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 20647-1-AP (ANO2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 20647-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20647-1-AP (ANO2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 20647-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20647-1-AP (ANO2 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: Y79 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse eye tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 3 publications below

Product Information

20647-1-AP targets ANO2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: anoctamin 2
• Calculated Molecular Weight: 114 kDa
• Observed Molecular Weight: 114 kDa
• GenBank Accession Number: NM_020373
• Gene Symbol: ANO2
• Gene ID (NCBI): 57101
• RRID: AB_10696181
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANO2, also named as C12orf3 and TMEM16B, belongs to the anoctamin family. It acts as a calcium-activated chloride channel (CaCC), mostly in photoreceptors. It may mediate olfactory amplification in olfactory sensory neurons (OSNs) and light perception amplification in retina.

Publications

Species	Application	Title
mouse,rat	IF	Nat Commun; Structural heterogeneity of the ion and lipid channel TMEM16F; Authors - Zhongjie Ye
mouse	IF	J Physiol; Functional expression of TMEM16A in taste bud cells.; Authors - Domenico M Guarascio ; KO Validated
mouse	WB	eNeuro; A role for STOML3 in olfactory sensory transduction.; Authors - Emilio Agostinelli
human	IF	Mol Cancer Res; Mechanisms of glioblastoma replication: Ca2+ flares and Cl- currents; Authors - Yunzhen Li