ANO4 Polyclonal antibody

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  WB analysis using 19488-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19488-1-AP (ANO4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ANO4 Polyclonal Antibody for WB, ELISA

Cat No. 19488-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

ANO4, anoctamin 4, TMEM16D, Transmembrane protein 16D

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

19488-1-AP targets ANO4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: anoctamin 4
• Calculated Molecular Weight: 111 kDa
• Observed Molecular Weight: 100-111 kDa
• GenBank Accession Number: NM_178826
• Gene Symbol: ANO4
• Gene ID (NCBI): 121601
• RRID: AB_3669309
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q32M45
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANO4, also named as TMEM16D, belongs to the anoctamin family. ANO4 is primarily expressed in the central nervous system and certain endocrine glands, and it has been linked to a variety of neurological disorders (PMID: 36836529). The antibody recognize the isoform1 and isoform2 of ANO4.

Publications

Species	Application	Title
human	IHC	Front Immunol; Gene Expression Profiling of Multiple Sclerosis Pathology Identifies Early Patterns of Demyelination Surrounding Chronic Active Lesions.; Authors - Debbie A E Hendrickx