Tested Applications
| Positive WB detected in | HeLa cells, mouse liver tissue, L02 cells |
| Positive IHC detected in | mouse liver tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20784-1-AP targets ANO6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13937 Product name: Recombinant human ANO6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-294 aa of BC098410 Sequence: MKKMSRNVLLQMEEEEDDDDGDIVLENLGQTIVPDLGSLESQHDFRTPEFEEFNGKPDSLFFNDGQRRIDFVLVYEDESRKETNKKGTNEKQRRKRQAYESNLICHGLQLEATRSVLDDKLVFVKVHAPWEVLCTYAEIMHIKLPLKPNDLKNRSSAFGTLNWFTKVLSVDESIIKPEQEFFTAPFEKNRMNDFYIVDRDAFFNPATRSRIVYFILSRVKYQVINNVSKFGINRLVNSGIYKAAFPLHDCKFRRQSEDPSCPNERYLLYREWAHPRSIYKKQPLDLIRKYYGEK Predict reactive species |
| Full Name | anoctamin 6 |
| Calculated Molecular Weight | 910 aa, 106 kDa |
| Observed Molecular Weight | 95 kDa |
| GenBank Accession Number | BC098410 |
| Gene Symbol | ANO6 |
| Gene ID (NCBI) | 196527 |
| RRID | AB_2878737 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q4KMQ2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Publications
What published studies show
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| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Cellular Adaptation to Mechanical Stress Emerges via Cell Shrinkage Triggered by Nonlinear Calcium Elevation. | ||
Life Sci Benzbromarone improves blood hypercoagulability after TBI by reducing phosphatidylserine externalization through inhibition of TMEM16F expression | ||
Metabolites Decreased Hyocholic Acid and Lysophosphatidylcholine Induce Elevated Blood Glucose in a Transgenic Porcine Model of Metabolic Disease | ||
Medicine (Baltimore) ANO6 is a reliable prognostic biomarker and correlates to macrophage polarization in breast cancer | ||
Open Med (Wars) ANO6 (TMEM16F) inhibits gastrointestinal stromal tumor growth and induces ferroptosis |
Reviews
What customers say
The product received one review with a 5-star rating. The reviewer used it for Western Blot on immortalized B lymphocytes at a 1:500 dilution and reported no issues, indicating satisfactory performance in their application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Christelle (Verified Customer) (04-22-2022) | nothing
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