Tested Applications
Positive WB detected in | HEK-293 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
19486-1-AP targets ANO7 in WB, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen |
fusion protein Predict reactive species |
Full Name | anoctamin 7 |
Calculated Molecular Weight | 105 kDa |
Observed Molecular Weight | 106 kDa |
GenBank Accession Number | NM_001001891 |
Gene Symbol | ANO7 |
Gene ID (NCBI) | 50636 |
RRID | AB_2878580 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q6IWH7 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ANO7, also named as NGEP, PCANAP5 and TMEM16G, belongs to the anoctamin family. It may act as a calcium-activated chloride channel and may play a role in cell-cell interactions.
Protocols
Product Specific Protocols | |
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WB protocol for ANO7 antibody 19486-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Vivienne (Verified Customer) (09-08-2025) | Lysates were prepared without cooking. 1x10^6 cells were incubated with RIPA buffer and sonified. After this BCA assay was performed for analysing the protein concentration. 50 µl plus 25 µl 3X Lämmli buffer + mercaptoethanol.
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