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Tested Applications
| Positive WB detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19486-1-AP targets ANO7 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | anoctamin 7 |
| Calculated Molecular Weight | 105 kDa |
| Observed Molecular Weight | 106 kDa |
| GenBank Accession Number | NM_001001891 |
| Gene Symbol | ANO7 |
| Gene ID (NCBI) | 50636 |
| RRID | AB_2878580 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6IWH7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ANO7, also named as NGEP, PCANAP5 and TMEM16G, belongs to the anoctamin family. It may act as a calcium-activated chloride channel and may play a role in cell-cell interactions.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ANO7 antibody 19486-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Vivienne (Verified Customer) (09-08-2025) | Lysates were prepared without cooking. 1x10^6 cells were incubated with RIPA buffer and sonified. After this BCA assay was performed for analysing the protein concentration. 50 µl plus 25 µl 3X Lämmli buffer + mercaptoethanol.
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