ANP32A Polyclonal antibody

• Featured Product
• KD/KO Validated

ANP32A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 15810-1-AP

Synonyms

MAPM, Leucine-rich acidic nuclear protein, LANP, I1PP2A, C15orf1

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Validation Data Gallery

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  WB analysis using 15810-1-AP

  Various cells were subjected to SDS PAGE followed by western blot with 15810-1-AP (ANP32A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15810-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15810-1-AP (ANP32A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 15810-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 15810-1-AP (ANP32A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15810-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15810-1-AP (ANP32A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15810-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15810-1-AP (ANP32A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15810-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15810-1-AP (ANP32A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 15810-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15810-1-AP (ANP32A Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 15810-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15810-1-AP (ANP32A Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of A431 using 15810-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A431 cells using 15810-1-AP (ANP32A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A2780 using 15810-1-AP

  Immunofluorescent analysis of A2780 cells, using ANP32A antibody 15810-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: A549 cells, human brain tissue, mouse brain tissue, mouse thymus tissue, Raji cells, HeLa cells, MCF-7 cells, HEK-293T cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells, A2780 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below

Product Information

15810-1-AP targets ANP32A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ANP32A fusion protein Ag8546
• Full Name: acidic (leucine-rich) nuclear phosphoprotein 32 family, member A
• Calculated Molecular Weight: 249 aa, 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC007200
• Gene Symbol: ANP32A
• Gene ID (NCBI): 8125
• RRID: AB_2056302
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANP32A, an acidic leucine-rich nuclear phosphoprotein, is a member of the inhibitor of the histone acetyltransferase (INHAT) complex and preferentially binds to unmodified histone H3 tails in vitro. ANP32A is expressed in normal tissues as well as in pancreatic, breast and prostate cancer and other malignant tumors. ANP32A plays an important role in cell proliferation, apoptosis, transcriptional regulation, signal transduction and other processes.

Publications

Species	Application	Title
human,chicken	WB	Sci Adv; Avian ANP32A incorporated in avian influenza A virions promotes interspecies transmission by priming early viral replication in mammals; Authors - Lei Na
human	WB	Cancer Cell Int; Super enhancer related gene ANP32B promotes the proliferation of acute myeloid leukemia by enhancing MYC through histone acetylation; Authors - Xiaomei Wan ; KD Validated
human	WB	Arch Virol; The interaction of cellular protein ANP32A with influenza A virus polymerase component PB2 promotes vRNA synthesis.; Authors - Xiuli Wei ; KD Validated