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  • KD/KO Validated

ANP32B Polyclonal antibody

ANP32B Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human and More (1)





Cat no : 10843-1-AP


ANP32B, APRIL, PHAPI2, Silver stainable protein SSP29, SSP29

Tested Applications

Positive WB detected inA431 cells, HEK-293 cells, HeLa cells, HEK-293T cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10843-1-AP targets ANP32B in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANP32B fusion protein Ag1274
Full Name acidic (leucine-rich) nuclear phosphoprotein 32 family, member B
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC013003
Gene Symbol ANP32B
Gene ID (NCBI) 10541
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANP32B is a member of the highly conserved acidic leucine-rich nuclear phosphoprotein 32 (ANP32) family. The ANP32 proteins have been implicated in a broad array of physiological processes, including cell differentiation, apoptotic cell death, and cell proliferation. ANP32B was identified as a novel direct substrate for caspase-3 and acted as a negative regulator for leukemic cell apoptosis. ANP32B controls the expression of the dendritic cell maturation factor CD83 by regulating the transport of its mRNA to the cytoplasm. It also modulates the activity of the transcription factor Kruppel-like factor 5 (KLF5).


Product Specific Protocols
WB protocol for ANP32B antibody 10843-1-APDownload protocol
IHC protocol for ANP32B antibody 10843-1-APDownload protocol
IF protocol for ANP32B antibody 10843-1-APDownload protocol
IP protocol for ANP32B antibody 10843-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Sci Adv

The SUMO-interacting motif in NS2 promotes adaptation of avian influenza virus to mammals

Authors - Liuke Sun

Proc Natl Acad Sci U S A

Acidic nuclear phosphoprotein 32kDa (ANP32)B-deficient mouse reveals a hierarchy of ANP32 importance in mammalian development.

Authors - Reilly Patrick T PT


Downregulation of ANP32B, a novel substrate of caspase-3, enhances caspase-3 activation and apoptosis induction in myeloid leukemic cells.

Authors - Shen Shao-Ming SM
  • KD Validated

Blood Cancer J

PMID: 24413065

Authors - B C Valdez

Cell Death Dis

ANP32B deficiency impairs proliferation and suppresses tumor progression by regulating AKT phosphorylation.

Authors - S Yang
  • KD Validated

J Virol

ANP32 proteins are essential for influenza virus replication in human cells.

Authors - Ecco Staller
  • KO Validated