ANUBL1 Polyclonal antibody, PBS Only

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• ANUBL1 Polyclonal antibody, PBS Only (17478-1-PBS)

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  WB analysis using 17478-1-AP (same clone as 17478-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17478-1-AP (ANUBL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17478-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 17478-1-AP (same clone as 17478-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17478-1-AP (ANUBL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17478-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 17478-1-AP (same clone as 17478-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17478-1-AP (ANUBL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17478-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 17478-1-AP (same clone as 17478-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17478-1-AP (ANUBL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17478-1-PBS in a different storage buffer formulation.

ANUBL1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 17478-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

17478-1-PBS targets ANUBL1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11505

  Product name: Recombinant human ANUBL1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 305-653 aa of BC048968

  Sequence: GNIVLPSEECVTEQSLLPKVGSLASFAEGNADEQSSGLEGACKVNLELLLTNADKGLKAPEQHLKHVAGVLNGESVETSVLNYRELSPHKNRLLSPLRCSAPMSLHNSLVKPERQSKCFEFGKLQPSSSQSLDVQNITDSSFSRTTCFQGVKVDSLGKRSDVISKVEARDITEMTNKASKEPVGCVNNISFLASLAGSTSRNRLQSTRGAGRLQNSGTGLSTNLQHFQEENFRKSSPQLEHTGVFLSTHGVGMNGNNAAAGKSVGECTTHHLPPVKAPLQTKKKTTNHCFLCGKKTGLASSYECRCGNNFCASHRYAETHGCTYDYKSAGRRYLHEANPVVNAPKLPKI

• Full Name: AN1, ubiquitin-like, homolog (Xenopus laevis)
• Calculated Molecular Weight: 653 aa, 72 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC048968
• Gene Symbol: ANUBL1
• Gene ID (NCBI): 93550
• RRID: AB_2923568
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86XD8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ANUBL1 is also named as ZFAND4. The expression of ANUBL1 was specifically upregulated in gastric cancer and it was positively associated with grades of gastric cancer. Moreover, it promoted proliferation of gastric cancer SGC-7901 cells. The mechanism of ANUBL1 upregulation in gastric cancer is that miR-182 suppressed the expression of ANUBL1 through targeting its 3'UTR, and ANUBL1 could downregulate the expression of anti-proliferation-associated miRNAs (miR-148b, miR-375 and miR-182) in SGC-7901 cells. The calculated molecular weight of ANUBL1 is 80 kDa (PMID: 25682742).