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Annexin A3 Polyclonal antibody

Annexin A3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11804-1-AP

Synonyms

35 alpha calcimedin, Annexin 3, annexin A3, Annexin III, ANX3, ANXA3, Lipocortin III, PAP III



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, mouse lung tissue, mouse testis tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman stomach cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11804-1-AP targets Annexin A3 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Annexin A3 fusion protein Ag2381
Full Name annexin A3
Calculated Molecular Weight 322 aa, 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC000871
Gene Symbol ANXA3
Gene ID (NCBI) 306
RRIDAB_2057455
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Annexin A3 (Anxa3), also named as lipocortin3, placental anticoagulant protein3 (PAP-III), belongs to annexin family. Annexins are calcium-dependent phospholipids-binding high-abundant intracellular proteins. ANXA3 has been reported to play crucial roles in a diverse range of molecular and cellular processes, including ion channel regulation, anticoagulation, anti-inflammation, vesicle traffick-ing, signal transduction, cell migration, and cell proliferation. It also exhibits important roles in cancer progression, metastasis, and drug resistance.

Protocols

Product Specific Protocols
WB protocol for Annexin A3 antibody 11804-1-APDownload protocol
IHC protocol for Annexin A3 antibody 11804-1-APDownload protocol
IP protocol for Annexin A3 antibody 11804-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseFC

Cancer Immunol Res

ANXA3-rich exosomes derived from tumor-associated macrophages regulate ferroptosis and lymphatic metastasis of laryngeal squamous cell carcinoma

Authors - Licheng Xu
humanIHC

Front Immunol

Upregulated of ANXA3, SORL1, and Neutrophils May Be Key Factors in the Progressionof Ankylosing Spondylitis.

Authors - Jie Jiang
ratIF

iScience

Deciphering the dynamic niches and regeneration-associated transcriptional program of motoneurons following peripheral nerve injury

Authors - Yu Zhang
mouseIF

Sci Rep

Characterization of slow cycling corneal limbal epithelial cells identifies putative stem cell markers.

Authors - R Sartaj
mouseIHC

Front Med (Lausanne)

An Integrated Proteomics and Metabolomics Strategy for the Mechanism of Calcium Oxalate Crystal-Induced Kidney Injury.

Authors - Songyan Gao
mouseWB,IHC

J Proteomics

Label-free proteomic analysis of placental proteins during Toxoplasma gondii infection.

Authors - Fang Jiao