|Positive WB detected in||HepG2 cells, HEK-293 cells, human brain tissue, human liver tissue, human testis tissue, mouse lung tissue, rat brain tissue|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||human stomach cancer tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11804-1-AP targets Annexin A3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Annexin A3 fusion protein Ag2381|
|Full Name||annexin A3|
|Calculated molecular weight||322 aa, 36 kDa|
|Observed molecular weight||36 kDa|
|GenBank accession number||BC000871|
|Gene ID (NCBI)||306|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Annexin A3 (Anxa3), also named as lipocortin3, placental anticoagulant protein3 (PAP-III), belongs to annexin family. Annexins are calcium-dependent phospholipids-binding high-abundant intracellular proteins. ANXA3 has been reported to play crucial roles in a diverse range of molecular and cellular processes, including ion channel regulation, anticoagulation, anti-inflammation, vesicle traffick-ing, signal transduction, cell migration, and cell proliferation. It also exhibits important roles in cancer progression, metastasis, and drug resistance.
J Adv Res
33-kDa ANXA3 isoform contributes to hepatocarcinogenesis via modulating ERK, PI3K/Akt-HIF and intrinsic apoptosis pathways.
Participation of delta annexin A3 in the ribosomal protein S19 C-terminus-dependent inhibitory mechanism of the neutrophil C5a receptor through delta lactoferrin.
Exp Mol Pathol
Annexin A3 plays a role in cytoplasmic calcium oscillation by extracellular calcium in the human promyelocytic leukemia HL-60 cells differentiated by phorbol-12-myristate-13-acetate.
A dynamic plasma membrane proteome analysis of alcohol-induced liver cirrhosis.
The roles of ribosomal protein S19 C-terminus in a shortened neutrophil lifespan through delta lactoferrin.
J Agric Food Chem
Effect of High-Fat Diet on Hepatic Proteomics of Hamsters.