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  • KD/KO Validated

Annexin A3 Polyclonal antibody

Annexin A3 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11804-1-AP

Synonyms

35 alpha calcimedin, Annexin 3, annexin A3, Annexin III, ANX3, ANXA3, Lipocortin III, PAP III



Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, human brain tissue, human liver tissue, human testis tissue, mouse lung tissue, rat brain tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman stomach cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11804-1-AP targets Annexin A3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Annexin A3 fusion protein Ag2381
Full Name annexin A3
Calculated molecular weight 322 aa, 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC000871
Gene symbol ANXA3
Gene ID (NCBI) 306
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Annexin A3 (Anxa3), also named as lipocortin3, placental anticoagulant protein3 (PAP-III), belongs to annexin family. Annexins are calcium-dependent phospholipids-binding high-abundant intracellular proteins. ANXA3 has been reported to play crucial roles in a diverse range of molecular and cellular processes, including ion channel regulation, anticoagulation, anti-inflammation, vesicle traffick-ing, signal transduction, cell migration, and cell proliferation. It also exhibits important roles in cancer progression, metastasis, and drug resistance.

Protocols

Product Specific Protocols
WB protocol for Annexin A3 antibody 11804-1-APDownload protocol
IHC protocol for Annexin A3 antibody 11804-1-APDownload protocol
IP protocol for Annexin A3 antibody 11804-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Adv Res

33-kDa ANXA3 isoform contributes to hepatocarcinogenesis via modulating ERK, PI3K/Akt-HIF and intrinsic apoptosis pathways.

Authors - Chunmei Guo
humanWB

Pathol Int

Participation of delta annexin A3 in the ribosomal protein S19 C-terminus-dependent inhibitory mechanism of the neutrophil C5a receptor through delta lactoferrin.

Authors - Koji Yamanegi
WB,IF

Exp Mol Pathol

Annexin A3 plays a role in cytoplasmic calcium oscillation by extracellular calcium in the human promyelocytic leukemia HL-60 cells differentiated by phorbol-12-myristate-13-acetate.

Authors - Hiroshi Nishiura
  • KD Validated
ratWB,IHC

Proteome Sci

A dynamic plasma membrane proteome analysis of alcohol-induced liver cirrhosis.

Authors - Jia Xiaofang X
humanWB

Immunobiology

The roles of ribosomal protein S19 C-terminus in a shortened neutrophil lifespan through delta lactoferrin.

Authors - Hiroshi Nishiura
ratWB

J Agric Food Chem

Effect of High-Fat Diet on Hepatic Proteomics of Hamsters.

Authors - Chen-Chung Liao