Annexin IV Polyclonal antibody

Annexin IV Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 10087-1-AP

Synonyms

35 beta calcimedin, Annexin 4, annexin A4, Annexin IV, ANX4, ANXA4, Chromobindin 4, Endonexin I, Lipocortin IV, P32.5, PAP II, PIG28, PP4 X, Protein II, ZAP36



Tested Applications

Positive WB detected inHuH-7 cells, RAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Sample-dependent, check data in validation data gallery

Product Information

10087-1-AP targets Annexin IV in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Annexin IV fusion protein Ag0130
Full Name annexin A4
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC000182
Gene symbol ANXA4
Gene ID (NCBI) 307
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Annexin IV /placental anticoagulant protein II (ANX4) is a member of the annexin or lipocortin family of calcium-dependent phospholipid binding proteins, and is located at 2p13. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. ANX4 shares 45 to 59% identity with other members and possibly interacts with ATP. ANX4 has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. ANX4 is almost exclusively expressed in epithelial cells. In western blotting, we get 36 kDa, 39 kDa(with modification), and 60 kDa( an unspecific band).

Protocols

Product Specific Protocols
WB protocol for Annexin IV antibody 10087-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Aging (Albany NY)

Membrane-cytoplasm translocation of annexin A4 is involved in the metastasis of colorectal carcinoma.

Authors - Ya Peng
humanWB

PLoS One

Quantitative proteomic analysis reveals that anti-cancer effects of selenium-binding protein 1 in vivo are associated with metabolic pathways.

Authors - Qi Ying
humanWB,IHC

Sci Rep

Anxa4 mediated airway progenitor cell migration promotes distal epithelial cell fate specification.

Authors - Kewu Jiang
humanWB

Mol Cell Proteomics

Proteomics Profiling of Autologous Blood and Semen Exosomes from HIV-infected and Uninfected Individuals Reveals Compositional and Functional Variabilities.

Authors - Hussein Kaddour
mouseWB

J Immunol

Pathogenic natural antibodies recognizing annexin IV are required to develop intestinal ischemia-reperfusion injury.

Authors - Kulik Liudmila L
humanWB

Mol Cell Proteomics

Proteomics analyses of human optic nerve head astrocytes following biomechanical strain.

Authors - Rogers Ronan S RS

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Yuan (Verified Customer) (09-30-2019)

The antibody is working very well on HeLa cells.

  • Applications: Western Blot,Immunoprecipitation,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HeLa