|Positive WB detected in||HuH-7 cells, RAW 264.7 cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Sample-dependent, check data in validation data gallery|
10087-1-AP targets Annexin IV in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Annexin IV fusion protein Ag0130|
|Full Name||annexin A4|
|Calculated molecular weight||36 kDa|
|Observed molecular weight||36 kDa|
|GenBank accession number||BC000182|
|Gene ID (NCBI)||307|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Annexin IV /placental anticoagulant protein II (ANX4) is a member of the annexin or lipocortin family of calcium-dependent phospholipid binding proteins, and is located at 2p13. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. ANX4 shares 45 to 59% identity with other members and possibly interacts with ATP. ANX4 has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. ANX4 is almost exclusively expressed in epithelial cells. In western blotting, we get 36 kDa, 39 kDa(with modification), and 60 kDa( an unspecific band).
Aging (Albany NY)
Membrane-cytoplasm translocation of annexin A4 is involved in the metastasis of colorectal carcinoma.
Quantitative proteomic analysis reveals that anti-cancer effects of selenium-binding protein 1 in vivo are associated with metabolic pathways.
Anxa4 mediated airway progenitor cell migration promotes distal epithelial cell fate specification.
Pathogenic natural antibodies recognizing annexin IV are required to develop intestinal ischemia-reperfusion injury.
Mol Cell Proteomics
Proteomics analyses of human optic nerve head astrocytes following biomechanical strain.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Yuan (Verified Customer) (09-30-2019)
The antibody is working very well on HeLa cells.