Validation Data Gallery
|Positive WB detected in||HeLa cells, human brain tissue, mouse brain tissue, mouse placenta tissue, mouse kidney tissue, A549 cells, Jurkat cells|
|Positive IP detected in||mouse kidney tissue|
|Positive IHC detected in||human colon tissue, human kidney tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
12542-1-AP targets Annexin VI in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Annexin VI fusion protein Ag3263|
|Full Name||annexin A6|
|Calculated molecular weight||673 aa, 76 kDa|
|Observed molecular weight||67 kDa|
|GenBank accession number||BC017046|
|Gene ID (NCBI)||309|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ANXA6, also called CBP68, or 67 kDa calelectrin, belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis.
|Product Specific Protocols|
|WB protocol for Annexin VI antibody 12542-1-AP||Download protocol|
|IHC protocol for Annexin VI antibody 12542-1-AP||Download protocol|
|IF protocol for Annexin VI antibody 12542-1-AP||Download protocol|
|IP protocol for Annexin VI antibody 12542-1-AP||Download protocol|
|Click here to view our Standard Protocols|
J Proteome Res
Significant Down-Regulation of Urea Cycle Generates Clinically Relevant Proteomic Signature in Hepatocellular Carcinoma Patients with Macrovascular Invasion.
Cell Death Dis
Exosomal annexin A6 induces gemcitabine resistance by inhibiting ubiquitination and degradation of EGFR in triple-negative breast cancer.
Onco Targets Ther
UNBS5162 inhibits proliferation of human retinoblastoma cells by promoting cell apoptosis.
A dynamic plasma membrane proteome analysis of alcohol-induced liver cirrhosis.
TMT-based proteomics analysis of the cerebral cortex of TauT knockout rats.
Front Cell Dev Biol
Dynamic Mitochondrial Proteome Under Polyamines Treatment in Cardiac Aging.