Annexin VI Polyclonal antibody

Annexin VI Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 12542-1-AP

Synonyms

67 kDa calelectrin, Annexin 6, annexin A6, Annexin VI, ANX6, ANXA6, Calphobindin II, CBP68, Chromobindin 20, CPB II, Lipocortin VI, p68, p70, Protein III



Tested Applications

Positive WB detected inHeLa cells, human brain tissue, mouse brain tissue, mouse placenta tissue, mouse kidney tissue, A549 cells, Jurkat cells
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman colon tissue, human kidney tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12542-1-AP targets Annexin VI in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Annexin VI fusion protein Ag3263
Full Name annexin A6
Calculated molecular weight 673 aa, 76 kDa
Observed molecular weight 67 kDa
GenBank accession numberBC017046
Gene symbol ANXA6
Gene ID (NCBI) 309
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ANXA6, also called CBP68, or 67 kDa calelectrin, belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis.

Protocols

Product Specific Protocols
WB protocol for Annexin VI antibody 12542-1-APDownload protocol
IHC protocol for Annexin VI antibody 12542-1-APDownload protocol
IF protocol for Annexin VI antibody 12542-1-APDownload protocol
IP protocol for Annexin VI antibody 12542-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

J Proteome Res

Significant Down-Regulation of Urea Cycle Generates Clinically Relevant Proteomic Signature in Hepatocellular Carcinoma Patients with Macrovascular Invasion.

Authors - Yin Cao
humanWB

Onco Targets Ther

UNBS5162 inhibits proliferation of human retinoblastoma cells by promoting cell apoptosis.

Authors - Bing Wang
ratWB

Proteome Sci

A dynamic plasma membrane proteome analysis of alcohol-induced liver cirrhosis.

Authors - Jia Xiaofang X
humanWB

Oncotarget

CD300A promotes tumor progression by PECAM1, ADCY7 and AKT pathway in acute myeloid leukemia.

Authors - Xiaogang Sun