|Positive WB detected in||HeLa cells, human brain tissue, mouse brain tissue, mouse placenta tissue, mouse kidney tissue, A549 cells, Jurkat cells|
|Positive IP detected in||mouse kidney tissue|
|Positive IHC detected in||human colon tissue, human kidney tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
12542-1-AP targets Annexin VI in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Annexin VI fusion protein Ag3263|
|Full Name||annexin A6|
|Calculated molecular weight||673 aa, 76 kDa|
|Observed molecular weight||67 kDa|
|GenBank accession number||BC017046|
|Gene ID (NCBI)||309|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ANXA6, also called CBP68, or 67 kDa calelectrin, belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis.
|Product Specific Protocols|
|WB protocol for Annexin VI antibody 12542-1-AP||Download protocol|
|IHC protocol for Annexin VI antibody 12542-1-AP||Download protocol|
|IF protocol for Annexin VI antibody 12542-1-AP||Download protocol|
|IP protocol for Annexin VI antibody 12542-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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