• Featured Product
  • KD/KO Validated

Annexin VI Polyclonal antibody

Annexin VI Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12542-1-AP

Synonyms

67 kDa calelectrin, Annexin 6, annexin A6, Annexin VI, ANX6, ANXA6, Calphobindin II, CBP68, Chromobindin 20, CPB II, Lipocortin VI, p68, p70, Protein III



Tested Applications

Positive WB detected inHeLa cells, human brain tissue, mouse brain tissue, mouse placenta tissue, mouse kidney tissue, A549 cells, Jurkat cells
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman colon tissue, human kidney tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12542-1-AP targets Annexin VI in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Annexin VI fusion protein Ag3263
Full Name annexin A6
Calculated Molecular Weight 673 aa, 76 kDa
Observed Molecular Weight 67 kDa
GenBank Accession NumberBC017046
Gene Symbol ANXA6
Gene ID (NCBI) 309
RRIDAB_2057753
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANXA6, also called CBP68, or 67 kDa calelectrin, belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis.

Protocols

Product Specific Protocols
WB protocol for Annexin VI antibody 12542-1-APDownload protocol
IHC protocol for Annexin VI antibody 12542-1-APDownload protocol
IF protocol for Annexin VI antibody 12542-1-APDownload protocol
IP protocol for Annexin VI antibody 12542-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Extracell Vesicles

Distinguishing functional exosomes and other extracellular vesicles as a nucleic acid cargo by the anion-exchange method.

Authors - Naohiro Seo
humanWB

Oncotarget

CD300A promotes tumor progression by PECAM1, ADCY7 and AKT pathway in acute myeloid leukemia.

Authors - Xiaogang Sun
ratWB

Front Cell Dev Biol

Dynamic Mitochondrial Proteome Under Polyamines Treatment in Cardiac Aging.

Authors - Hao Zhang
ratWB,IF

Proteome Sci

TMT-based proteomics analysis of the cerebral cortex of TauT knockout rats.

Authors - Yiming Xia
ratWB

Proteome Sci

A dynamic plasma membrane proteome analysis of alcohol-induced liver cirrhosis.

Authors - Jia Xiaofang X
humanWB

Onco Targets Ther

UNBS5162 inhibits proliferation of human retinoblastoma cells by promoting cell apoptosis.

Authors - Bing Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Gaurav (Verified Customer) (02-29-2024)

Very good antibody with expected band at appropriate position. Works well in TBST, 5% Skimmed milk and 3% BSA in TBST

  • Applications: Western Blot, Immunoprecipitation, Cell culture
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: Smooth Muscle cells, Aorta, Placental Trophoblasts.