ANXA9 Polyclonal antibody

ANXA9 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC

Conjugate

Unconjugated

Cat no : 15416-1-AP

Synonyms

Annexin 31, Annexin 9, annexin A9, Annexin XXXI, ANX31, ANXA9, Pemphaxin

---

Validation Data Gallery

• 

  WB analysis using 15416-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15416-1-AP (ANXA9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 15416-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15416-1-AP (ANXA9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15416-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15416-1-AP (ANXA9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 15416-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15416-1-AP (ANXA9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 15416-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15416-1-AP (ANXA9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 15416-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15416-1-AP (ANXA9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 15416-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15416-1-AP (ANXA9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 15416-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15416-1-AP (ANXA9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 15416-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15416-1-AP (ANXA9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 15416-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15416-1-AP (ANXA9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 15416-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15416-1-AP (ANXA9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 15416-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15416-1-AP (ANXA9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 15416-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15416-1-AP (ANXA9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 15416-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15416-1-AP (ANXA9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 15416-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15416-1-AP (ANXA9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 15416-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15416-1-AP (ANXA9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 15416-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15416-1-AP (ANXA9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 15416-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15416-1-AP (ANXA9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15416-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15416-1-AP (ANXA9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: A431 cells, MCF-7 cells
• Positive IHC detected in: human breast cancer tissue, human colon cancer tissue, human heart tissue, human kidney tissue, human liver tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15416-1-AP targets ANXA9 in WB, IHC applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ANXA9 fusion protein Ag7702
• Full Name: annexin A9
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC005830
• Gene Symbol: ANXA9
• Gene ID (NCBI): 8416
• RRID: AB_2057905
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.