AOAH Polyclonal antibody

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  WB analysis of mouse colon using 12911-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 12911-1-AP (AOAH Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 12911-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 12911-1-AP (AOAH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 12911-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12911-1-AP (AOAH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 12911-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12911-1-AP (AOAH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 12911-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12911-1-AP (AOAH Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 12911-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12911-1-AP (AOAH Antibody) at dilution of 1:50 (under 40x lens).

AOAH Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12911-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

Acyloxyacyl hydrolase, Acyloxyacyl hydrolase large subunit, Acyloxyacyl hydrolase small subunit, EC:3.1.1.77

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Tested Applications

• Positive WB detected in: mouse colon tissue, mouse spleen tissue
• Positive IHC detected in: mouse colon tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IP: See 1 publications below

Product Information

12911-1-AP targets AOAH in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3892

  Product name: Recombinant human AOAH protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 226-575 aa of BC025698

  Sequence: QDSNCNGIWGVDPKDGVPYEKKFCEGSQPRGIILLGDSAGAHFHISPEWITASQMSLNSFINLPTALTNELDWPQLSGATGFLDSTVGIKEKSIYLRLWKRNHCNHRDYQNISRNGASSRNLKKFIESLSRNKVLDYPAIVIYAMIGNDVCSGKSDPVPAMTTPEKLYSNVMQTLKHLNSHLPNGSHVILYGLPDGTFLWDNLHNRYHPLGQLNKDMTYAQLYSFLNCLQVSPCHGWMSSNKTLRTLTSERAEQLSNTLKKIAASEKFTNFNLFYMDFAFHEIIQEWQKRGGQPWQLIEPVDGFHPNEVALLLLADHFWKKVQLQWPQILGKENPFNPQIKQVFGDQGGH

• Full Name: acyloxyacyl hydrolase (neutrophil)
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC025698
• Gene Symbol: AOAH
• Gene ID (NCBI): 313
• RRID: AB_2877891
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P28039
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Acyloxyacyl hydrolase (AOAH) is a highly conserved lipase responsible for hydrolyzing the secondary fatty acyl chains of gram-negative bacterial lipopolysaccharides (LPS), resulting in LPS detoxification and attenuation of host inflammation (PMID: 1577781, PMID: 35980963). AOAH has also been shown to have PLA2 function (PMID: 1577781). AOAH was highly expressed at the protein level in lesional psoriatic skin compared to healthy controls (PMID: 34913478). AOAH has been described to be differentially proteolytically processed into both a secreted and cytosolic form in phagocytic cells (PMID: 19755182). Expression of AOAH is primarily attributed to phagocytic cells such as neutrophils, macrophages, DC, and also NK cells (PMID: 33454018).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB,IP	Science; Enterically derived high-density lipoprotein restrains liver injury through the portal vein.; Authors - Yong-Hyun Han