Tested Applications
| Positive WB detected in | mouse lung tissue, mouse adipose tissue, rat lung tissue, mouse stomach tissue |
| Positive IHC detected in | human appendicitis tissue, human lung cancer tissue, human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14365-1-AP targets VAP1/AOC3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5683 Product name: Recombinant human AOC3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 408-763 aa of BC050549 Sequence: ATYVDWHFLLESQAPKTIRDAFCVFEQNQGLPLRRHHSDLYSHYFGGLAETVLVVRSMSTLLNYDYVWDTVFHPSGAIEIRFYATGYISSAFLFGATGKYGNQVSEHTLGTVHTHSAHFKVDLDVAGLENWVWAEDMVFVPMAVPWSPEHQLQRLQVTRKLLEMEEQAAFLVGSATPRYLYLASNHSNKWGHPRGYRIQMLSFAGEPLPQNSSMARGFSWERYQLAVTQRKEEEPSSSSVFNQNDPWAPTVDFSDFINNETIAGKDLVAWVTAGFLHIPHAEDIPNTVTVGNGVGFFLRPYNFFDEDPSFYSADSIYFRGDQDAGACEVNPLACLPQAAACAPDLPAFSHGGFSHN Predict reactive species |
| Full Name | amine oxidase, copper containing 3 (vascular adhesion protein 1) |
| Calculated Molecular Weight | 85 kDa |
| Observed Molecular Weight | 85-90 kDa |
| GenBank Accession Number | BC050549 |
| Gene Symbol | AOC3 |
| Gene ID (NCBI) | 8639 |
| RRID | AB_2058043 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16853 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
AOC3(membrane primary amine oxidase) is also name as copper amine oxidase, HPAO, SSAO, VAP1 and belongs to the copper/topaquinone oxidase family. It is expressed on the surface of endothelial cells and is involved in leukocyte trafficking between blood and tissues under physiologic and pathologic conditions (PMID:17947691). AOC3 is highly expressed in adipocytes and smooth muscle cells and it can be N- and O-glycosylated (PMID:16046623). AOC3 has molecular mass of 70-90kD, and can exsit as a homodimer(165-185kD) and trimer(240~260kD) (PMID:10595925, 8625974).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for VAP1/AOC3 antibody 14365-1-AP | Download protocol |
| WB protocol for VAP1/AOC3 antibody 14365-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The VAP1/AOC3 Polyclonal antibody (Cat# 14365-1-AP) has 2 citations. One appears in Experimental and Molecular Medicine (2026), studying cardiac fibrosis and PDGFR signaling in myofibroblasts using WB in mouse. The other is in Journal of Cellular and Molecular Medicine (2022), investigating single-cell transcriptional profiling of human carotid plaques and stroke-associated endothelial subpopulations using IHC in human. The research fields span cardiovascular biology, fibrosis, and stroke pathology.
| Species | Application | Title |
|---|---|---|
Exp Mol Med VAP1 promotes cardiac fibrosis by enabling PDGFR signaling in myofibroblasts. | ||
J Cell Mol Med Single-cell transcriptional profiling of human carotid plaques reveals a subpopulation of endothelial cells associated with stroke incidences. |
Reviews
What customers say
The single review for this product (mouse liver lysate, Western Blot, 1:1000 dilution) rates it 4 out of 5. The reviewer reported the correct band size and a clean background, but noted the signal was relatively weak. Overall, the antibody performs as expected in terms of specificity, though sensitivity could be improved.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Hua (Verified Customer) (10-21-2020) | Right size band and Clean background, but with relatively weak signal.
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