Tested Applications
| Positive WB detected in | mouse liver tissue, mouse lung tissue, mouse pancreas tissue |
| Positive IP detected in | mouse lung tissue |
| Positive IHC detected in | human hepatocirrhosis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19495-1-AP targets Aldehyde oxidase in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.
| Tested Reactivity | human, mouse, rat, canine |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | aldehyde oxidase 1 |
| Calculated Molecular Weight | 148 kDa |
| Observed Molecular Weight | 148 kDa |
| GenBank Accession Number | NM_001159 |
| Gene Symbol | AOX1 |
| Gene ID (NCBI) | 316 |
| RRID | AB_10640579 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q06278 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Aldehyde oxidase antibody 19495-1-AP | Download protocol |
| IHC protocol for Aldehyde oxidase antibody 19495-1-AP | Download protocol |
| IP protocol for Aldehyde oxidase antibody 19495-1-AP | Download protocol |
| WB protocol for Aldehyde oxidase antibody 19495-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Aldehyde oxidase Polyclonal antibody (Cat# 19495-1-AP) has 18 citations spanning journals including Gut Microbes, Oncogene, EMBO Reports, Free Radical Biology and Medicine, Cell Signaling, Frontiers in Immunology, and Stem Cell Reports. Research fields cover NAFLD, diabetic nephropathy, bladder and gallbladder cancer, lung adenocarcinoma, renal cell carcinoma, glioblastoma, IBD, ethanol metabolism, drug-induced hepatotoxicity, osteogenic differentiation, fibrosis, placental aging, and neurogenic niche biology. Applications are predominantly WB and IHC.
| Species | Application | Title |
|---|---|---|
Gut Microbes Time-restricted feeding ameliorates non-alcoholic fatty liver disease through modulating hepatic nicotinamide metabolism via gut microbiota remodeling | ||
Phytomedicine Integrated metabolomics and mass spectrometry imaging analysis reveal the efficacy and mechanism of Huangkui capsule on type 2 diabetic nephropathy | ||
Oncogene Epigenetic loss of AOX1 expression via EZH2 leads to metabolic deregulations and promotes bladder cancer progression.
| ||
Arch Pharm Res Protective effects of L-theanine on rats with dextran sulfate sodium-induced inflammatory bowel disease. | ||
Free Radic Biol Med PPARα agonist WY-14,643 enhances ethanol metabolism in mice: role of catalase. | ||
Reviews
What customers say
Both reviewers report positive results. One user (5/5) found the antibody effective across Western Blot, IHC, IF, and IP at a 1:1000 dilution on breast and prostate cancer tissues, calling it a great product. The other (4/5) achieved a clean 148 kDa signal on rat brain lysate by WB at 1:500 dilution using a 10% gel and 0.45 µm PVDF membrane. Overall, users highlight strong specificity, clean bands, and versatility across multiple applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Vasudevarao (Verified Customer) (11-14-2025) | This antibody works very well for the all type of application. Great product from Proteinetch
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MJ (Verified Customer) (05-27-2025) | Clean signal at 148kDA on rat brain lysate using 10% gel on 0.45um PVDF diluted in Hikari Signal Enhancer
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