Aldehyde oxidase Polyclonal antibody

• Featured Product
• KD/KO Validated

Aldehyde oxidase Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 19495-1-AP

Synonyms

EC:1.2.3.1, EC:1.17.3.-, Azaheterocycle hydroxylase, AOX1, AOX 1

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Validation Data Gallery

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  WB analysis using 19495-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19495-1-AP (Aldehyde oxidase antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 19495-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19495-1-AP (Aldehyde oxidase antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 19495-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 19495-1-AP (Aldehyde oxidase antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 19495-1-AP

  IP result of anti-Aldehyde oxidase (IP:19495-1-AP, 4ug; Detection:19495-1-AP 1:300) with mouse lung tissue lysate 4000ug.

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  IHC staining of human hepatocirrhosis using 19495-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 19495-1-AP (Aldehyde oxidase antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 19495-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 19495-1-AP (Aldehyde oxidase antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 19495-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Aldehyde oxidase antibody (19495-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: mouse liver tissue, mouse lung tissue, mouse pancreas tissue
• Positive IP detected in: mouse lung tissue
• Positive IHC detected in: human hepatocirrhosis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

19495-1-AP targets Aldehyde oxidase in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

• Tested Reactivity: human, mouse, rat, canine
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: aldehyde oxidase 1
• Calculated Molecular Weight: 148 kDa
• Observed Molecular Weight: 148 kDa
• GenBank Accession Number: NM_001159
• Gene Symbol: AOX1
• Gene ID (NCBI): 316
• RRID: AB_10640579
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	EMBO Rep; Deficiency in mTORC1-controlled C/EBPβ-mRNA translation improves metabolic health in mice.; Authors - Laura M Zidek
human	WB,IHC	Oncogene; Epigenetic loss of AOX1 expression via EZH2 leads to metabolic deregulations and promotes bladder cancer progression.; Authors - Venkatrao Vantaku ; KD Validated
human	IF	Am J Obstet Gynecol; Evidence that fetal death is associated with placental aging.; Authors - Kaushik Maiti
mouse	WB	Free Radic Biol Med; PPARα agonist WY-14,643 enhances ethanol metabolism in mice: role of catalase.; Authors - Xue Chen
human	WB	Front Immunol; Insight of a Metabolic Prognostic Model to Identify Tumor Environment and Drug Vulnerability for Lung Adenocarcinoma.; Authors - Shun-Li Peng
human	WB	Cell Signal; Aldehyde oxidase 1 promotes gallbladder carcinogenesis through ROS-mediated activation of the Wnt/β-catenin pathway; Authors - Ziheng Yuan