AP repeat Polyclonal antibody

AP repeat Polyclonal Antibody for ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 24493-1-AP

Synonyms

AP repeat, APAPAPAPA, C9RAN, GGGCC, poly AP



Tested Applications

Positive ELISA detected inpeptide

Recommended dilution

ApplicationDilution
Enzyme-linked Immunosorbent Assay (ELISA)ELISA : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

24493-1-AP targets AP repeat in WB, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name AP repeat
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The C9orf72 "GGGGCC" repeat sequence codes five repeat peptide "GA repeat; GAGAGAGAGA", "GP repeat; GPGPGPGPG", "GR repeat; GRGRGRGRG", "AP repeat; APAPAPAPA" and "PR repeat; PRPRPRPRP". It was described previously that aggregated forms of poly-GA and poly-GP proteins do not enter the separation gel (PMID: 26374446). This antibody is used to detect the "AP repeat" sequence. This antibody detects the APAPAPAPA peptide with dilution 1:32,000 in Elisa.

Publications

SpeciesApplicationTitle
humanWB

J Cell Sci

SFPQ regulates the accumulation of RNA foci and dipeptide repeat proteins from the expanded repeat mutation in C9orf72.

Authors - Mirjana Malnar
humanWB,IF

Hum Mol Genet

Modelling C9orf72 dipeptide repeat proteins of a physiologically relevant size.

Authors - Janis Bennion Callister
humanIF

Acta Neuropathol

Antisense RNA foci in the motor neurons of C9ORF72-ALS patients are associated with TDP-43 proteinopathy.

Authors - Johnathan Cooper-Knock
humanWB, IHC

Acta Neuropathol

Quantitative analysis and clinico-pathological correlations of different dipeptide repeat protein pathologies in C9ORF72 mutation carriers.

Authors - Ian R A Mackenzie
humanIHC

Acta Neuropathol

Increased prevalence of granulovacuolar degeneration in C9orf72 mutation.

Authors - Yuichi Riku