Tested Applications
| Positive ELISA detected in | peptide |
Recommended dilution
| Application | Dilution |
|---|---|
| Enzyme-linked Immunosorbent Assay (ELISA) | ELISA : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24493-1-AP targets AP repeat in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | AP repeat |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2879572 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The C9orf72 "GGGGCC" repeat sequence codes five repeat peptide "GA repeat; GAGAGAGAGA", "GP repeat; GPGPGPGPG", "GR repeat; GRGRGRGRG", "AP repeat; APAPAPAPA" and "PR repeat; PRPRPRPRP". It was described previously that aggregated forms of poly-GA and poly-GP proteins do not enter the separation gel (PMID: 26374446). This antibody is used to detect the "AP repeat" sequence. This antibody detects the APAPAPAPA peptide with dilution 1:32,000 in Elisa.
Publications
What published studies show
The AP repeat Polyclonal antibody (Cat# 24493-1-AP) has 9 citations across seven journals: Acta Neuropathologica (3), Cell Reports, FASEB Journal, Journal of Cell Science, Cancer Medicine, Human Molecular Genetics, and Life Science Alliance. Research fields include C9ORF72 repeat expansion and ALS/neurodegeneration, dipeptide repeat protein pathology, neurodevelopment, synovial chondromatosis, and SIRT1 expression in gastrointestinal tumors. Applications cited include WB, IHC, and IF in human samples.
| Species | Application | Title |
|---|---|---|
Acta Neuropathol Increased prevalence of granulovacuolar degeneration in C9orf72 mutation. | ||
Acta Neuropathol Antisense RNA foci in the motor neurons of C9ORF72-ALS patients are associated with TDP-43 proteinopathy. | ||
Acta Neuropathol Quantitative analysis and clinico-pathological correlations of different dipeptide repeat protein pathologies in C9ORF72 mutation carriers. | ||
FASEB J Cellular Landscape of Synovial Chondromatosis Synovium Revealed by Single-Cell RNA Sequencing | ||
J Cell Sci SFPQ regulates the accumulation of RNA foci and dipeptide repeat proteins from the expanded repeat mutation in C9orf72. |
