AP2A1 Polyclonal antibody

AP2A1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 29887-1-AP

Synonyms

ADTAA, Alpha adaptin A, Alpha1 adaptin, AP 2 complex subunit alpha 1, AP2 ALPHA, AP2A1, CLAPA1

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Validation Data Gallery

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  WB analysis using 29887-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29887-1-AP (AP2A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 29887-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 29887-1-AP (AP2A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 29887-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 29887-1-AP (AP2A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 29887-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using AP2A1 antibody (29887-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of SH-SY5Y using 29887-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using AP2A1 antibody (29887-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, SH-SY5Y cells, mouse heart tissue
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: SH-SY5Y cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29887-1-AP targets AP2A1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AP2A1 fusion protein Ag31809
• Full Name: adaptor-related protein complex 2, alpha 1 subunit
• Calculated Molecular Weight: 108KD
• Observed Molecular Weight: 100-108 kDa
• GenBank Accession Number: NM_014203
• Gene Symbol: AP2A1
• Gene ID (NCBI): 160
• RRID: AB_2918351
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AP2A1 encodes adaptor protein complex 2 (AP-2) subunit alpha-1, which is a 100 kDa coated vesicle protein. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP complexes are involved in the formation of clathrin-coated vesicles (CCVs) by recruiting the scaffold protein, clathrin. AP complexes also play a pivotal role in the cargo selection by recognizing the sorting signals within the cytoplasmic tail of integral membrane proteins. AP-2 is involved in clathrin-dependent endocytosis.