AP2A2 Polyclonal antibody

AP2A2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15722-1-AP

Synonyms

ADTAB, Alpha adaptin C, Alpha2 adaptin, AP 2 complex subunit alpha 2, AP2A2, CLAPA2, HIP 9, HIP9, Huntingtin yeast partner J, HYPJ, KIAA0899

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Validation Data Gallery

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  WB analysis of mouse brain using 15722-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15722-1-AP (AP2A2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 15722-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 15722-1-AP (AP2A2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 15722-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15722-1-AP (AP2A2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 15722-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15722-1-AP (AP2A2 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

15722-1-AP targets AP2A2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AP2A2 fusion protein Ag8442
• Full Name: adaptor-related protein complex 2, alpha 2 subunit
• Calculated Molecular Weight: 939 aa, 104 kDa
• Observed Molecular Weight: 104 kDa
• GenBank Accession Number: BC006155
• Gene Symbol: AP2A2
• Gene ID (NCBI): 161
• RRID: AB_2878175
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AP2A2, also named as ADTAB, CLAPA2, HIP9, HYPJ, or KIAA0899, is a 939 amino acid protein, which belongs to the adaptor complexes large subunit family. AP2A2 localizes in the membrane and is a component of the adaptor protein complex 2. AP2 adaptor complex linking clathrin to receptors in coated vesicles, clathrin-associated protein complexes are believed to interact with cytoplasmic domains of transmembrane proteins leading to their selection and concentration. AP2A2 is invovled into cellular trafficking transport.

Publications

Species	Application	Title
pig	WB	Emerg Microbes Infect; CD1d facilitates African swine fever virus entry into the host cells via clathrin-mediated endocytosis; Authors - Xin Chen