AP2A2 Polyclonal antibody

AP2A2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 15722-1-AP

Synonyms

ADTAB, Alpha adaptin C, Alpha2 adaptin, AP 2 complex subunit alpha 2, AP2A2, CLAPA2, HIP 9, HIP9, Huntingtin yeast partner J, HYPJ, KIAA0899



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

15722-1-AP targets AP2A2 in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AP2A2 fusion protein Ag8442
Full Name adaptor-related protein complex 2, alpha 2 subunit
Calculated molecular weight 939 aa, 104 kDa
Observed molecular weight 104 kDa
GenBank accession numberBC006155
Gene symbol AP2A2
Gene ID (NCBI) 161
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AP2A2, also named as ADTAB, CLAPA2, HIP9, HYPJ, or KIAA0899, is a 939 amino acid protein, which belongs to the adaptor complexes large subunit family. AP2A2 localizes in the membrane and is a component of the adaptor protein complex 2. AP2 adaptor complex linking clathrin to receptors in coated vesicles, clathrin-associated protein complexes are believed to interact with cytoplasmic domains of transmembrane proteins leading to their selection and concentration. AP2A2 is invovled into cellular trafficking transport.

Protocols

Product Specific Protocols
WB protocol for AP2A2 antibody 15722-1-APDownload protocol
IHC protocol for AP2A2 antibody 15722-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols