AP4B1 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 84710-3-RR (same clone as 84710-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84710-3-RR (AP4B1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84710-3-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 84710-3-RR (same clone as 84710-3-PBS)

  IP result of anti-AP4B1 (IP:84710-3-RR, 4ug; Detection:84710-3-RR 1:1500) with HeLa cells lysate 1200 ug. This data was developed using the same antibody clone with 84710-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84710-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84710-3-RR against Human AP4B1 were performed. The affinity constant is 47.1 pM.

AP4B1 Uni-rAb^® Recombinant Antibody for WB, IP, Indirect ELISA

Cat No. 84710-3-PBS

Clone No.242121D4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, Indirect ELISA

Synonyms

Beta4-adaptin, Beta subunit of AP-4, AP-4 complex subunit beta-1, AP-4 adaptor complex subunit beta, Adaptor-related protein complex 4 subunit beta-1

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Product Information

84710-3-PBS targets AP4B1 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag9348

  Product name: Recombinant human AP4B1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-351 aa of BC014146

  Sequence: MPYLGSEDVVKELKKALCNPHIQADRLRYRNVIQRVIRYMTQGLDMSGVFMEMVKASATVDIVQKKLVYLYMCTYAPLKPDLALLAINTLCKDCSDPNPMVRGLALRSMCSLRMPGVQEYIQQPILNGLRDKASYVRRVAVLGCAKMHNLHGDSEVDGALVNELYSLLRDQDPIVVVNCLRSLEEILKQEGGVVINKPIAHHLLNRMSKLDQWGQAEVLNFLLRYQPRSEEELFDILNLLDSFLKSSSPGVVMGATKLFLILAKMFPHVQTDVLVRVKGPLLAACSSESRELCFVALCHVRQILHSLPGHFSSHYKKFFCSYSEPHYIKLQKVEVLCELVNDENVQQVLEE

• Full Name: adaptor-related protein complex 4, beta 1 subunit
• Calculated Molecular Weight: 739 aa, 83 kDa
• Observed Molecular Weight: ~75 kDa
• GenBank Accession Number: BC014146
• Gene Symbol: AP4B1
• Gene ID (NCBI): 10717
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9Y6B7
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Adaptor protein (AP) complex is a component of intracellular protein transport. The AP4 complex is involved in sorting transmembrane cargo proteins into transport vesicles for trans-Golgi network export. AP4B1 is a member of the AP4 complex, which also includes AP4E1, AP4M1 and AP4S1. Loss-of-function variants in any of these four members can lead to AP-4-associated hereditary spastic paraplegia (HSP), a neurodegenerative disorder (PMID: 30543385). This antibody recognizes AP4B1.