AP4M1 Polyclonal antibody

AP4M1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 11653-1-AP

Synonyms

AP 4 complex subunit mu 1, AP4M1, MU 4, Mu adaptin related protein 2, MU ARP2, Mu subunit of AP 4, mu4, Mu4 adaptin, MUARP2

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Validation Data Gallery

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  WB analysis of mouse heart using 11653-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11653-1-AP (AP4M1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 11653-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11653-1-AP (AP4M1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11653-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11653-1-AP (AP4M1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 11653-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 11653-1-AP (AP4M1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 11653-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11653-1-AP (AP4M1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 11653-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11653-1-AP (AP4M1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse heart tissue, HeLa cells, mouse lung tissue, A431 cells
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

11653-1-AP targets AP4M1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AP4M1 fusion protein Ag2222
• Full Name: adaptor-related protein complex 4, mu 1 subunit
• Calculated Molecular Weight: 453 aa, 50 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC018705
• Gene Symbol: AP4M1
• Gene ID (NCBI): 9179
• RRID: AB_2227267
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Adaptor protein (AP) complex is a component of intracellular protein transport. The AP4 complex is involved in sorting transmembrane cargo proteins into transport vesicles for trans-Golgi network export. AP4M1 is a member of the AP4 complex, which also includes AP4B1, AP4E1, and AP4S1. Loss-of-function variants in any of these four members can lead to AP-4-associated hereditary spastic paraplegia (HSP), a neurodegenerative disorder (PMID: 30543385). This antibody recognizes AP4M1.

Publications

Species	Application	Title
human	WB	Cancer Cell Int; AP4M1 as a prognostic biomarker associated with cell proliferation, migration and immune regulation in hepatocellular carcinoma; Authors - Yuanhao Peng