|Positive WB detected in||human kidney tissue|
|Positive IHC detected in||human liver tissue, human spleen tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 20773-1-AP is Serum amyloid P component Fusion Protein expressed in E. coli.
|Host / Isotype||Rabbit / IgG|
|Immunogen||Serum amyloid P component fusion protein Ag14765|
|Full Name||amyloid P component, serum|
|Calculated molecular weight||223 aa, 25 kDa|
|Observed molecular weight||27-29 kDa|
|GenBank accession number||BC007058|
|Gene ID (NCBI)||325|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Serum amyloid P component (SAP), a member of pentraxin family, is a constitutive serum protein that is synthesized by hepatocytes. It is an acute phase protein, structurally related to C-reactive protein. SAP is a calcium-dependent ligand binding protein, which can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1.
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