Serum amyloid P component Polyclonal antibody

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• Serum amyloid P component Polyclonal antibody (20773-1-AP)

Serum amyloid P component Polyclonal Antibody for WB, IHC, ELISA

Cat No. 20773-1-AP Copied!

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

APCS, SAP, 9.5S alpha 1 glycoprotein, 9.5S alpha-1-glycoprotein, amyloid P component, serum

• 

  WB analysis of human kidney using 20773-1-AP

  Human kidney tissue was subjected to SDS PAGE followed by western blot with 20773-1-AP (Serum amyloid P component antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 20773-1-AP

  Human plasma was subjected to SDS PAGE followed by western blot with 20773-1-AP (Serum amyloid P component antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 20773-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20773-1-AP (Serum amyloid P component antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 20773-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20773-1-AP (Serum amyloid P component antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human spleen using 20773-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 20773-1-AP (Serum amyloid P component antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human spleen using 20773-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 20773-1-AP (Serum amyloid P component antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 20773-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 20773-1-AP (Serum amyloid P component antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: human plasma, human kidney tissue
• Positive IHC detected in: human liver tissue, human spleen tissue, human normal colon; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20773-1-AP targets Serum amyloid P component in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14765

  Product name: Recombinant human APCS protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 20-223 aa of BC007058

  Sequence: HTDLSGKVFVFPRESVTDHVNLITPLEKPLQNFTLCFRAYSDLSRAYSLFSYNTQGRDNELLVYKERVGEYSLYIGRHKVTSKVIEKFPAPVHICVSWESSSGIAEFWINGTPLVKKGLRQGYFVEAQPKIVLGQEQDSYGGKFDRSQSFVGEIGDLYMWDSVLPPENILSAYQGTPLPANILDWQALNYEIRGYVIIKPLVWV

• Full Name: amyloid P component, serum
• Calculated Molecular Weight: 223 aa, 25 kDa
• Observed Molecular Weight: 27-29 kDa
• GenBank Accession Number: BC007058
• Gene Symbol: Serum amyloid P component
• Gene ID (NCBI): 325
• RRID: AB_10915553
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P02743
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Serum amyloid P component (SAP), a member of pentraxin family, is a constitutive serum protein that is synthesized by hepatocytes. It is an acute phase protein, structurally related to C-reactive protein. SAP is a calcium-dependent ligand binding protein, which can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.

Publications

What published studies show

The Serum amyloid P component (SAP) polyclonal antibody (Cat# 20773-1-AP) has a total of 2 citations. These appear in JACC Basic Transl Sci (2023) and Sci Rep (2020). The associated research fields include bioprosthetic valve deterioration involving circulating protein accumulation and macrophage infiltration, as well as CRP-mediated recognition of uric acid crystals and recruitment of proteases C1 and MASP1, spanning cardiovascular pathology and innate immune response.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	JACC Basic Transl Sci; Bioprosthetic Valve Deterioration: Accumulation of Circulating Proteins and Macrophages in the Valve Interstitium; Authors - Tomohisa Sakaue
human	WB	Sci Rep; C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1.; Authors - Anika Alberts