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APEX1 Monoclonal antibody

APEX1 Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig, Rabbit

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

2B10B2

Cat no : 67781-1-Ig

Synonyms

AP endonuclease 1, APE, APE1, APE1,APEX1, APEN, APEX, APEX nuclease, APEX1, APX, HAP1, Protein REF 1, REF1



Tested Applications

Positive WB detected inJurkat cells, HeLa cells, pig brain tissue, LNCaP cells, HEK-293 cells, HepG2 cells, mouse brain tissue, rabbit brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Product Information

67781-1-Ig targets APEX1 in WB,ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.

Tested Reactivity Human, Mouse, Rat, Pig, Rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen APEX1 fusion protein Ag28552
Full Name APEX nuclease (multifunctional DNA repair enzyme) 1
Calculated molecular weight 36 kDa
Observed molecular weight36 kDa
GenBank accession numberBC002338
Gene symbol APEX1
Gene ID (NCBI) 328
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

APEX1, also named as APE, APE1, HAP1 and REF-1, belongs to the DNA repair enzymes AP/ExoA family. It is a multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 are in DNA repair and redox regulation of transcriptional factors. APEX nuclease is a DNA repair enzyme having apurinic/apyrimidinic (AP) endonuclease, 3-prime,5-prime-exonuclease, DNA 3-prime repair diesterase, and DNA 3-prime-phosphatase activities. On the other hand, APEX1 also exerts reversible nuclear redox activity to regulate DNA binding affinity and transcriptional activity of transcriptional factors by controlling the redox status of their DNA-binding domain, such as the FOS/JUN AP-1 complex after exposure to IR. APEX1 is involved in calcium-dependent down-regulation of parathyroid hormone (PTH) expression by binding to negative calcium response elements (nCaREs). When acetylated at Lys-6 and Lys-7, APEX1 stimulates the YBX1-mediated MDR1 promoter activity, leading to drug resistance. It also acts as an endoribonuclease involved in the control of single-stranded RNA metabolism. It plays a role in regulating MYC mRNA turnover by preferentially cleaving in between UA and CA dinucleotides of the MYC coding region determinant (CRD). In association with NMD1, APEX1 plays a role in the rRNA quality control process during cell cycle progression.

Protocols

Product Specific Protocols
WB protocol for APEX1 antibody 67781-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols