APIP Recombinant monoclonal antibody

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  WB analysis using 83320-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83320-5-RR (APIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83320-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83320-5-RR against Human APIP were performed. The affinity constant is 5.86 nM.

APIP Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 83320-5-RR

Clone No.240189E2

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

APAF1 interacting protein, APIP, APIP2, CGI 29, CGI29, dJ179L10.2, MMRP19, MTRu 1 P dehydratase

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Tested Applications

• Positive WB detected in: PC-3 cells, K-562 cells，

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83320-5-RR targets APIP in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag2729

  Product name: Recombinant human APIP protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-242 aa of BC009077

  Sequence: MSGCDAREGDCCSRRCGAQDKEHPRYLIPELCKQFYHLGWVTGTGGGISLKHGDEIYIAPSGVQKERIQPEDMFVCDINEKDISGPSPSKKLKKSQCTPLFMNAYTMRGAGAVIHTHSKAAVMATLLFPGREFKITHQEMIKGIKKCTSGGYYRYDDMLVVPIIENTPEEKDLKDRMAHAMNEYPDSCAVLVRRHGVYVWGETWEKAKTMCECYDYLFDIAVSMKKVGLDPSQLPVGENGIV

• Full Name: APAF1 interacting protein
• Calculated Molecular Weight: 242 aa, 27 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC009077
• Gene Symbol: APIP
• Gene ID (NCBI): 51074
• RRID: AB_3670986
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q96GX9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.