APLF Polyclonal antibody

APLF Polyclonal Antibody for WB, ELISA

Cat No. 14252-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

APFL, APLF, Aprataxin and PNK like factor, aprataxin and PNKP like factor, C2orf13, PALF, Xip1, XRCC1 interacting protein 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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WB analysis of PC-3 using 14252-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 14252-1-AP (APLF antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of PC-3 using 14252-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 14252-1-AP (APLF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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What's the best dilution for APLF in ?

Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in

PC-3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14252-1-AP targets APLF in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5525 Product name: Recombinant human APLF protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 162-511 aa of BC041144 Sequence: SVSFLGENRDCNKQQPILAERKRILPTWMLAEHLSDQNLSVPAISGGNVIQGSGKEEICKDKSQLNTTQQGRRQLISSGSSENTSAEQDTGEECKNTDQEESTISSKEMPQSFSAITLSNTEMNNIKTNAQRNKLPIEELGKVSKHKIATKRTPHKEDEAMSCSENCSSAQGDSLQDESQGSHSESSSNPSNPETLHAKATDSVLQGSEGNKVKRTSCMYGANCYRKNPVHFQHFSHPGDSDYGGVQIVGQDETDDRPECPYGPSCYRKNPQHKIEYRHNTLPVRNVLDEDNDNVGQPNEYDLNDSFLDDEEEDYEPTDEDSDWEPGKEDEEKEDVEELLKEAKRFMKRK Predict reactive species
Full Name	aprataxin and PNKP like factor
Calculated Molecular Weight	57 kDa
Observed Molecular Weight	60 kDa
GenBank Accession Number	BC041144
Gene Symbol	APLF
Gene ID (NCBI)	200558
RRID	AB_10641990
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8IW19
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for APLF antibody 14252-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The APLF Polyclonal antibody (Cat# 14252-1-AP) has 1 citation. It was used in a study published in Nature Structural & Molecular Biology (IF 10.1, 2019) titled "Mechanism for recycling tRNAs on stalled ribosomes." The research field is ribosome biology and translation regulation, specifically examining how tRNAs are recycled from stalled ribosomes.