APOB Polyclonal antibody

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  WB analysis using 27751-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27751-1-AP (APOB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27751-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27751-1-AP (APOB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 27751-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27751-1-AP (APOB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 27751-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27751-1-AP (APOB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

APOB Polyclonal Antibody for WB, IHC, ELISA

Cat No. 27751-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Apolipoprotein B, Apo B, Apo B-100, ApoB100, ApoB48

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Tested Applications

• Positive WB detected in: mouse liver tissue, HepG2 cells
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27751-1-AP targets APOB in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag26773

  Product name: Recombinant human APOB protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 23-220 aa of NM_000384

  Sequence: MAGARAEEEMLENVSLVCPKDATRFKHLRKYTYNYEAESSSGVPGTADSRSATRINCKVELEVPQLCSFILKTSQCTLKEVYGFNPEGKALLKKTKNSEEFAAAMSRYELKLAIPEGKQVFLYPEKDEPTYILNIKRGIISALLVPPETEEAKQVLFLDTVYGNCSTHFTVKTRKGNVATEISTERDLGQCDRFKPIRT

• Full Name: apolipoprotein B (including Ag(x) antigen)
• Calculated Molecular Weight: 516 kDa
• Observed Molecular Weight: 150-250 kDa, 400-520 kDa
• GenBank Accession Number: NM_000384
• Gene Symbol: APOB
• Gene ID (NCBI): 338
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P04114
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The apolipoprotein B (APOB) is a plasma protein synthesized primarily in the liver and intestine and play an important role in lipid and cholesterol metabolism. The APOB encodes two different isoproteins through mRNA editing, APOB-48 and APOB-100. APOB-48 and APOB-100 is present in both human liver and intestine. APOB-100 is essential for the assembly of VLDL in the liver. APOB-48 is essential for the assembly of chylomicrons in the intestine. It is well established that APOB-100 levels are associated with coronary heart disease. This antibody recognizes both of APOB-48, APOB-100 (PMID:11839763, PMID:2450346).