APOB48R Polyclonal antibody

• 

  WB analysis using 25328-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25328-1-AP (APOB48R antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

APOB48R Polyclonal Antibody for WB, ELISA

Cat No. 25328-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

apoB 48R, apoB-48R, APOBR, Apolipoprotein B 100 receptor, Apolipoprotein B 48 receptor

---

Tested Applications

• Positive WB detected in: mouse testis tissue, rat testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25328-1-AP targets APOB48R in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18204

  Product name: Recombinant human APOB48R protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 851-1088 aa of BC119786

  Sequence: SQTARAEGMGAMVEAGGLLEKWTLLEEEAVGWQEREQREDSEGRCGDYHPEGEAPRLLDAEGLMVTGGRRAEAKETEPESLEHVRGQEEQPTHQAPAEAAPESVGEAETAEAMGSARGGAANSWSEAPLPGSLLDVSVPRSRVHLSRSSSQRRSRPSFRRTPAWEQQEEPPAPNPPEEELSAPEQRPLQLEEPLEPSPLRHDGTPVPARRRPLGHGFGLAHPGMMQELQARLGRPKPQ

• Full Name: apolipoprotein B48 receptor
• Calculated Molecular Weight: 1088 aa, 116 kDa
• Observed Molecular Weight: 116 kDa
• GenBank Accession Number: BC119786
• Gene Symbol: APOB48R
• Gene ID (NCBI): 55911
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q0VD83
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Apolipoprotein B48 receptor (APOB48R) is a macrophage receptor that binds to apoB of dietary triglyceride-rich lipoproteins or to a similar apoB-like domain in hypertriglyceridemic very low-density lipoprotein (HTG-VLDL). This receptor is crucial for the uptake of chylomicrons, HTG-VLDL, and trypsinized VLDL lacking apoE in macrophages. APOB48R may contribute necessary lipids, lipid-soluble vitamins, and other nutrients to reticuloendothelial cells. Elevated plasma triglyceride levels may cause APOB48R to be a factor in foam cell formation, endothelial dysfunction, and atherothrombogenesis (PMID: 10852956; 9633939; 15591219).