APOBEC2 Polyclonal antibody

APOBEC2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 20121-1-AP

Synonyms

APOBEC2, ARCD1, ARP1

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Validation Data Gallery

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  WB analysis using 20121-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20121-1-AP (APOBEC2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 20121-1-AP

  IP result of anti-APOBEC2 (IP:20121-1-AP, 4ug; Detection:20121-1-AP 1:500) with mouse skeletal muscle tissue lysate 4000ug.

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  IHC staining of mouse heart using 20121-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20121-1-AP (APOBEC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 20121-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20121-1-AP (APOBEC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 20121-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20121-1-AP (APOBEC2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 20121-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20121-1-AP (APOBEC2 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse skeletal muscle tissue, rat heart tissue, rat skeletal muscle tissue
• Positive IP detected in: mouse skeletal muscle tissue
• Positive IHC detected in: mouse heart tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20121-1-AP is APOBEC2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: APOBEC2 fusion protein Ag13699
• Full Name: apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 2
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 25-30 kDa
• GenBank Accession Number: BC047767
• Gene Symbol: APOBEC2
• Gene ID (NCBI): 10930
• RRID: AB_10697687
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

APOBEC2, also named as ARCD1 and ARP1, is a member of the activation-induced deaminase/apolipoprotein B mRNA editing enzyme catalytic polypeptide (APOBEC) cytidine deaminase family expressed in differentiated skeletal and cardiac muscle. APOBEC family members have diverse roles by their ability to edit DNA and/or RNA. Apobec2 is one of the oldest members of the APOBEC family, along with the lymphoid-specific activation-induced deaminase. Unlike other APOBEC family members, the enzymatic activity and substrate of Apobec2 have not been fully demonstrated, and its biologic functions remain unknown. It can be detected as 25-30 kDa.

Publications

Species	Application	Title
		Int J Oncol; HBV triggers APOBEC2 expression through miR‑122 regulation and affects the proliferation of liver cancer cells.; Authors - Aimei Li
		J Dermatol Sci; Proteomic changes related to actin cytoskeleton function in the skin of vildagliptin-treated mice; Authors - Antti Nätynki