Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14559-1-AP targets APOBEC3B in WB, IHC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6115 Product name: Recombinant human APOBEC3B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-251 aa of BC053859 Sequence: MNPQIRNPMERMYRDTFYDNFENEPILYGRSYTWLCYEVKIKRGRSNLLWDTGVFRGQVYFEPQYHAEMCFLSWFCGNQLPAYKCFQITWFVSWTPCPDCVAKLAEFLSEHPNVTLTISAARLYYYWERDYRRALCRLSQAGARVKIMDYEEFAYCWENFVYNEGQQFMPWYKFDENYAFLHRTLKEILRLRIFSVAFTAAMRSCASWTWFLLCSWTRPRSTGSLGSSPGAPASPGAVPGKCVRSFRRTHT Predict reactive species |
| Full Name | apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3B |
| Calculated Molecular Weight | 46 kDa |
| Observed Molecular Weight | 46 kDa |
| GenBank Accession Number | BC053859 |
| Gene Symbol | APOBEC3B |
| Gene ID (NCBI) | 9582 |
| RRID | AB_2878066 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UH17 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The DNA cytosine deaminase APOBEC3B was identified recently as a source of DNA damage and mutagenesis in breast, head/neck, cervix, bladder, lung, ovary, and to lesser extents additional cancer types. This enzyme is also an effector protein in the innate immune response to virus infection. APOBEC3B has 3 isoforms produced by alternative splicing with the MW of 46, 29, 43 kDa. It can also exist as a dimer and can interact with APOBEC3G.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for APOBEC3B antibody 14559-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The APOBEC3B Polyclonal Antibody (Cat# 14559-1-AP) has 7 citations appearing in Nucleic Acids Research, BMC Medicine, Molecular Carcinogenesis, Journal of Medical Genetics, Frontiers in Oncology, Endocrine, and Zhongguo Fei Ai Za Zhi. Associated research fields include virology (HBV), oncology (NSCLC, cervical cancer, ovarian cancer, glioma, adrenocortical carcinoma), and genomic pathogenic analysis.
| Species | Application | Title |
|---|---|---|
BMC Med Genomic pathogenic alterations in the SWI/SNF complex compromise the outcomes of immunotherapy in Chinese patients with KRAS-mutant NSCLC by downregulating STING expression. | ||
Mol Carcinog HPV-16/18 E6-induced APOBEC3B expression associates with proliferation of cervical cancer cells and hypomethylation of Cyclin D1. | ||
J Med Genet Multiregion whole-genome sequencing depicts intratumour heterogeneity and punctuated evolution in ovarian clear cell carcinoma. | ||
Front Oncol Correlation Between APOBEC3B Expression and Clinical Characterization in Lower-Grade Gliomas. | ||
Endocrine Machine Learning-Driven Identification and In Vitro Validation of the APOBEC3B-ANLN Regulatory Axis in Adrenocortical Carcinoma.
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