APOBEC3C Polyclonal antibody

• Featured Product
• KD/KO Validated

APOBEC3C Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 10591-1-AP

Synonyms

ARDC2, APOBEC3, APOBEC1-like, APOBEC1L, APOBEC

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Validation Data Gallery

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  WB analysis of HEK-293 using 10591-1-AP

  WB result of APOBEC3C antibody (10591-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-APOBEC3C transfected HEK-293 cells.

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  WB analysis using 10591-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10591-1-AP (APOBEC3C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 10591-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 10591-1-AP (APOBEC3C antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 10591-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10591-1-AP (APOBEC3C Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 10591-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10591-1-AP (APOBEC3C Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 10591-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10591-1-AP (APOBEC3C Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 10591-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10591-1-AP (APOBEC3C Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Caco-2 cells, HEK-293 cells, K-562 cells, SW480 cells
• Positive IHC detected in: human ovary cancer tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:600-1:2400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

10591-1-AP targets APOBEC3C in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: APOBEC3C fusion protein Ag0886
• Full Name: apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3C
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC011739
• Gene Symbol: APOBEC3C
• Gene ID (NCBI): 27350
• RRID: AB_2057393
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

APOBEC3C(apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3C) is also named as APOBEC1L, PBI and belongs to the cytidine and deoxycytidylate deaminase family.APOBEC3C is related to APOBEC1, a cytidine deaminase responsible for cytidine-to-uridine editing of apolipoprotein B (APOB)mRNA.It can form a dimer(PMID:19074429).

Publications

Species	Application	Title
mouse	WB	J Exp Med; APOBEC3A catalyzes mutation and drives carcinogenesis in vivo.; Authors - Emily K Law
human	WB	Nucleic Acids Res; Enterovirus 71 antagonizes the inhibition of the host intrinsic antiviral factor A3G.; Authors - Zhaolong Li
human	IHC	Oncogenesis; Aberrant APOBEC3C expression induces characteristic genomic instability in pancreatic ductal adenocarcinoma.; Authors - Yunzhen Qian
human	IHC	Sci Rep; Expression and subcellular localisation of AID and APOBEC3 in adenoid and palatine tonsils.; Authors - Noriko Seishima
human	WB	J Virol; Infection of Bronchial Epithelial Cells by the Human Adenoviruses A12, B3, and C2 Differently Regulates the Innate Antiviral Effector APOBEC3B.; Authors - Noémie Lejeune
human	WB	Retrovirology; Natural APOBEC3C variants can elicit differential HIV-1 restriction activity.; Authors - Brett D Anderson