APOE Recombinant monoclonal antibody, PBS Only (Detector)

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  Sandwich ELISA standard curve of MP01558-3

  Sandwich ELISA standard curve of MP01558-3, Human APOE Recombinant Matched Antibody Pair - PBS only. 84775-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0448. 84775-4-PBS was HRP conjugated as the detection antibody. Range: 0.313-10 ng/mL.

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  Sample test of MP01558-3

  Serum of six individual healthy human donors was measured. The APOE concentration of detected samples was determined to be 25,140.92 ng/mL with a range of 9,706.32-52,603.90 ng/mL.

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  Sample test of MP01558-3

  Saliva of six individual healthy human donors was measured. The APOE concentration of detected samples was determined to be 8.16 ng/mL with a range of 3.43-16.91 ng/mL.

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  Sample test of MP01558-3

  Urine of six individual healthy human donors was measured. The APOE concentration of detected samples was determined to be 3.88 ng/mL with a range of 0.26-12.28 ng/mL.

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  Sample test of MP01558-3

  Human milk of six individual healthy human donors was measured. The APOE concentration of detected samples was determined to be 392.90 ng/mL with a range of 291.94-474.33 ng/mL.

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  Sample test of MP01558-3

  HepG2 were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human APOE, and measured 264.90 ng/mL.

APOE Uni-rAb^® Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84775-4-PBS

Clone No.242310A3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

ApolipoproteinE, apolipoprotein E, apoe4, Apo-E, Apo E

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Product Information

84775-4-PBS targets APOE as part of a matched antibody pair:

MP01558-3: 84775-5-PBS capture and 84775-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: Apolipoprotein E
• Calculated Molecular Weight: 36 kDa
• GenBank Accession Number: BC003557
• Gene Symbol: APOE
• Gene ID (NCBI): 348
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P02649
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.