APOM Polyclonal antibody

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  WB analysis using 12817-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12817-1-AP (APOM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human plasma using 12817-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 12817-1-AP (APOM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 12817-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12817-1-AP (APOM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 12817-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12817-1-AP (APOM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 12817-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 12817-1-AP (APOM Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human hepatocirrhosis using 12817-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 12817-1-AP (APOM Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 12817-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using APOM antibody (12817-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

APOM Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 12817-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Apo M, Apolipoprotein M, Apo-M, G3A, HSPC336

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Tested Applications

• Positive WB detected in: HepG2 cells, human plasma
• Positive IHC detected in: human liver tissue, human hepatocirrhosis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

12817-1-AP targets APOM in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3137

  Product name: Recombinant human APOM protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-188 aa of BC020683

  Sequence: MFHQIWAALLYFYGIILNSIYQCPEHSQLTTLGVDGKEFPEVHLGQWYFIAGAAPTKEELATFDPVDNIVFNMAAGSAPMQLHLRATIRMKDGLCVPRKWIYHLTEGSTDLRTEGRPDMKTELFSSSCPGGIMLNETGQGYQRFLLYNRSPHPPEKCVEEFKSLTSCLDSKAFLLTPRNQEACELSNN

• Full Name: apolipoprotein M
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 21-25 kDa
• GenBank Accession Number: BC020683
• Gene Symbol: APOM
• Gene ID (NCBI): 55937
• RRID: AB_2877884
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O95445
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

APOM (Apolipoprotein M) is a member of the lipocalin superfamily and is predominantly associated with high-density lipoprotein (HDL). APOM is synthesized in the kidneys, liver, and to a minor extent also by adipocytes. In the circulation, APOM binds primarily to high-density lipoproteins (HDL) but can also be associated with other lipoproteins, such as low-density lipoproteins (LDL), very-low-density lipoproteins (VLDL), and chylomicrons (PMID: 34482091).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Apoptosis; The miR-573/apoM/Bcl2A1-dependent signal transduction pathway is essential for hepatocyte apoptosis and hepatocarcinogenesis.; Authors - Yan-Wei Hu
mouse	WB	J Biol Chem; Effect of Phospholipid Transfer Protein on Plasma Sphingosine-1-Phosphate; Authors - Quiana Jones ; KO Validated