APP Recombinant antibody, PBS Only (Detector)

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  WB analysis of fetal human brain using 84829-6-RR (same clone as 84829-6-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 84829-6-RR (APP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84829-6-PBS in a different storage buffer formulation.

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  Sample test of MP01584-4

  The mean APP concentration was determined to be 151.0 ng/mL in human serum.

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  IF Staining of U-251 using 84829-6-RR (same clone as 84829-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using APP antibody (84829-6-RR, Clone: 250546B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84829-6-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01584-4

  Cytometric bead array standard curve of MP01584-4, APP Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84829-7-PBS. Detection antibody: 84829-6-PBS. Standard: Eg3070. Range: 0.625-80 ng/mL.

APP Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test

Cat No. 84829-6-PBS

Clone No.250546B3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test

Synonyms

amyloid precursor protein, A4 amyloid protein, AB40, AB42, AD1

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Product Information

84829-6-PBS targets APP as part of a matched antibody pair:

MP01584-4: 84829-7-PBS capture and 84829-6-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: APP fusion protein Eg3070
• Full Name: amyloid beta (A4) precursor protein
• Calculated Molecular Weight: 87 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: NM_000484.3
• Gene Symbol: APP
• Gene ID (NCBI): 351
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P05067-1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Aβ derives from APP via proteolytic cleavage by proteases called α-, β- and γ-secretase. The α-secretase cleavage precludes the formation of Aβ, while the β- and γ-cleavages generate APP components with amyloidogenic features. Amyloid beta A4 precursor protein(APP), encoded by APP gene which locate on human chromosome 21q, is a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. APP expressed in all fetal tissues and is pronounced in brain, kidney, heart and spleen, but weak in liver. Defects in APP are the cause of Alzheimer disease type 1 (AD1). Amyloid β (Aβ) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms. APP can be cleaved into several chains, this antibody could recognize N-terminal fragment of APP (N-APP).