APPL1 Polyclonal antibody
APPL1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 19885-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human brain tissue, HEK-293 cells, HeLa cells, HT-1080 cells, human heart tissue, mouse brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Product Information
19885-1-AP targets APPL1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | APPL1 fusion protein Ag13703 |
Full Name | adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 |
Calculated molecular weight | 709 aa, 80 kDa |
Observed molecular weight | 80 kDa |
GenBank accession number | BC028599 |
Gene symbol | APPL1 |
Gene ID (NCBI) | 26060 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), a binding partner of Akt2 and an important regulator of ins signaling, plays a key role in the regulation of ins secretion [PMID:22615370]. APPL1 interacts with adiponectin receptors and mediates the ins-sensitizing effects of adiponectin in muscle and endothelial cells. It also participates in nuclear signaling and transcriptional regulation, mostly by modulating the activity of various nuclear factors [PMID:22685329]. Apart from its role in endocytosis and endosomal transport, APPL1 was reported to undergo nucleocytoplasmic shuttling and participate in transcriptional regulation, e.g. by interactions with histone deacetylases (HDACs) [PMID:19686092].
Protocols
Product Specific Protocols | |
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WB protocol for APPL1 antibody 19885-1-AP | Download protocol |
IHC protocol for APPL1 antibody 19885-1-AP | Download protocol |
IF protocol for APPL1 antibody 19885-1-AP | Download protocol |
IP protocol for APPL1 antibody 19885-1-AP | Download protocol |
FC protocol for APPL1 antibody 19885-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |