AQP1 Monoclonal antibody

AQP1 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

2E5D6

Cat no : 66805-1-Ig

Synonyms

AQP 1, AQP CHIP, AQP1, Aquaporin 1, Aquaporin CHIP, CHIP28, CO, Urine water channel



Tested Applications

Positive WB detected inhuman heart tissue, human skeletal muscle tissue, pig kidney tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

66805-1-Ig targets AQP1 in WB, IHC,ELISA applications and shows reactivity with Human, pig samples.

Tested Reactivity Human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AQP1 fusion protein Ag14093
Full Name aquaporin 1 (Colton blood group)
Calculated molecular weight 269 aa, 29 kDa
Observed molecular weight 28 kDa, 38-40 kDa
GenBank accession numberBC022486
Gene symbol AQP1
Gene ID (NCBI) 358
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AQP1 is a member of aquaporins (AQPs) that are small membrane-spanning proteins facilitating water transport. AQP1 is expressed in most tissues in the mammalian body. Alterations of AQP1 expression have been linked to variety of diseases, including cancer. The predicted molecular weight of AQP1 is around 28 kDa, while highly glycosylated form can also be observed around 40-45 kDa. (1530176)

Protocols

Product Specific Protocols
WB protocol for AQP1 antibody 66805-1-IgDownload protocol
IHC protocol for AQP1 antibody 66805-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols