AQR Polyclonal antibody

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  WB analysis of HEK-293 using 24342-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24342-1-AP (AQR Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 24342-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24342-1-AP (AQR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 24342-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24342-1-AP (AQR Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 24342-1-AP

  IP result of anti-AQR (IP:24342-1-AP, 4ug; Detection:24342-1-AP 1:1000) with HEK-293 cells lysate 2800ug.

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  IHC staining of human kidney using 24342-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 24342-1-AP (AQR Antibody) at dilution of 1:50.

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  IHC staining of human brain using 24342-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 24342-1-AP (AQR Antibody) at dilution of 1:50.

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  IHC staining of human brain using 24342-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 24342-1-AP (AQR Antibody) at dilution of 1:50.

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  IHC staining of human kidney using 24342-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 24342-1-AP (AQR Antibody) at dilution of 1:50.

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  IF Staining of U2OS using 24342-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using AQR antibody (24342-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  IF Staining of U2OS using 24342-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using AQR antibody (24342-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

AQR Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 24342-1-AP

Publications(2)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

KIAA0560, Intron-binding protein of 160 kDa, IBP160, fSAP164, EC:3.6.4.13

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human brain tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• CoIP: See 1 publications below

Product Information

24342-1-AP targets AQR in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17810

  Product name: Recombinant human AQR protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1136-1485 aa of BC127111

  Sequence: QGRARASLCNLYNWRYKNLGNLPHVQLLPEFSTANAGLLYDFQLINVEDFQGVGESEPNPYFYQNLGEAEYVVALFMYMCLLGYPADKISILTTYNGQKHLIRDIINRRCGNNPLIGRPNKVTTVDRFQGQQNDYILLSLVRTRAVGHLRDVRRLVVAMSRARLGLYIFARVSLFQNCFELTPAFSQLTARPLHLHIIPTEPFPTTRKNGERPSHEVQIIKNMPQMANFVYNMYMHLIQTTHHYHQTLLQLPPAMVEEGEEVQNQETELETEEEAMTVQADIIPSPTDTSCRQETPAFQTDTTPSETGATSTPEAIPALSETTPTVVGAVSAPAEANTPQDATSAPEETK

• Full Name: aquarius homolog (mouse)
• Calculated Molecular Weight: 1485 aa, 171 kDa
• Observed Molecular Weight: 160-171 kDa
• GenBank Accession Number: BC127111
• Gene Symbol: AQR
• Gene ID (NCBI): 9716
• RRID: AB_2879501
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O60306
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,CoIP	Sci Adv; FAM50A drives breast cancer brain metastasis through interaction with C9ORF78 to enhance ʟ-asparagine production; Authors - Weilong Chen
		Mol Cell; Efficient Pre-mRNA Cleavage Prevents Replication-Stress-Associated Genome Instability.; Authors - Federico Teloni

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Roy (Verified Customer) (06-15-2024)

Did work perfectly to detect Aquarius by WB in HeLa extracts from HeLa cells. (1/1000 dilution, Overnight incubation at 4°C)

• Applications: Western Blot
• Cell Tissue Type: HeLa