ARAP1 Polyclonal antibody

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• ARAP1 Polyclonal antibody (17541-1-AP)

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  WB analysis using 17541-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17541-1-AP (ARAP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 17541-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17541-1-AP (ARAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 17541-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17541-1-AP (ARAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 17541-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17541-1-AP (ARAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ARAP1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 17541-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1, Centaurin-delta-2, CENTD2, Cnt-d2, KIAA0782

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Tested Applications

• Positive WB detected in: HepG2 cells, K-562 cells, L02 cells
• Positive IHC detected in: human kidney tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17541-1-AP targets ARAP1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11597

  Product name: Recombinant human ARAP1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 329-679 aa of BC056401

  Sequence: LYCVQCFSDTNQMNVHNLAIVFGPTLFQTDGQDYKAGRVVEDLINHYVVVFSVDEEELRKQREEITAIVKMRVAGTASGTQHAGDFICTVYLEEKKAETEQHIKVPASMTAEELTLEILDRRNVGIREKDYWTCFEVNEREEAERPLHFAEKVLPILHGLGTDSHLVVKKHQAMEAMLLYLASRVGDTKHGMMKFREDRSLLGLGLPSGGFHDRYFILNSSCLRLYKEVRSHRPEKEWPIKSLKVYLGVKKKLRPPTCWGFTVVHETEKHEKQQWYLCCDTQMELREWFATFLFVQHDGLVWPSEPSRMSRAVPEVRLGSVSLIPLRGSENEMRRSVAAFTADPLSLLRNV

• Full Name: ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1
• Calculated Molecular Weight: 162 kDa
• Observed Molecular Weight: 180 kDa
• GenBank Accession Number: BC056401
• Gene Symbol: ARAP1
• Gene ID (NCBI): 116985
• RRID: AB_3741923
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96P48
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARAP1 is a member of ARAP family with both Arf GAP and Rho GAP activities, by which ARAP1 makes a direct link between ADP-ribosylation factor (Arf) GTPases and Rho GTPases signaling. ARAP1 is involved in membrane trafficking and receptor recycling. It supports the plasma membrane recycling of the angiotensin II type 1 receptor (AT1) and regulates the cell-specific trafficking of the pro-death receptor TRAIL-R1/DR4, thereby promoting TRAIL-induced apoptosis in certain cell types. (PMID: 14559250, PMID: 18165900, PMID: 38008882)