• Featured Product
  • KD/KO Validated

ARFGAP1 Polyclonal antibody

ARFGAP1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13571-1-AP

Synonyms

ARF GAP 1, ARF1 GAP, ARF1GAP, ARFGAP1, HRIHFB2281



Tested Applications

Positive WB detected inPC-3 cells, HeLa cells, human brain tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13571-1-AP targets ARFGAP1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARFGAP1 fusion protein Ag4468
Full Name ADP-ribosylation factor GTPase activating protein 1
Calculated Molecular Weight 414 aa, 46 kDa
Observed Molecular Weight 46 kDa, 50 kDa
GenBank Accession NumberBC028233
Gene Symbol ARFGAP1
Gene ID (NCBI) 55738
RRIDAB_2058621
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein coats deform flat lipid membranes into buds and capture membrane proteins to form transport vesicles. The assembly-disassembly cycle of the COPI coat on Golgi membranes is coupled to the GTP-GDP cycle of the small G protein ARF1. ARFGAP1 contributes to vesicle budding by increasing the GTPase activity of ARF1, because it promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. for ARFGAP1 may has a role in regulating the GTPase activity and neuronal toxicity of LRRK2; reciprocally, LRRK2 phosphorylates ArfGAP1 and is required for ArfGAP1 neuronal toxicity.

Protocols

Product Specific Protocols
WB protocol for ARFGAP1 antibody 13571-1-APDownload protocol
IHC protocol for ARFGAP1 antibody 13571-1-APDownload protocol
IF protocol for ARFGAP1 antibody 13571-1-APDownload protocol
IP protocol for ARFGAP1 antibody 13571-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

BioE3 identifies specific substrates of ubiquitin E3 ligases

Authors - Orhi Barroso-Gomila
mouse,ratWB,IF

PLoS Genet

GTPase activity and neuronal toxicity of Parkinson's disease-associated LRRK2 is regulated by ArfGAP1.

Authors - Klodjan Stafa
  • KD Validated
humanWB, IF

FASEB J

ArfGAP1 acts as a GTPase-activating protein for human ADP-ribosylation factor-like 1 protein.

Authors - Hsiang-Pu Feng
  • KD Validated
human,pigWB

Vet Microbiol

ARFGAP1 binds to classical swine fever virus NS5A protein and enhances CSFV replication in PK-15 cells.

Authors - Liang Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Boyan (Verified Customer) (10-27-2021)

This antibody clearly label the Golgi localisation of endogenous ArfGAP1, and also gives a clean signal in WB.

  • Applications: Western Blot, Immunofluorescence
FH

Cristian (Verified Customer) (04-17-2019)

Immunofluorescent analysis of (PFA 3%) fixed HeLa cells using 13571-1-AP (ARFGAP1 antibody) at dilution of 1:500 and Alexa Fluor 594-conjugated Affinipure Goat Anti-Rabbit IgG (H+L)

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: HeLa
ARFGAP1 Antibody Immunofluorescence, validation (1:500 dilution) in HeLa (Cat no:13571-1-AP)