ARFGAP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ARFGAP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (2)

Cat no : 13571-1-AP

Synonyms

ARF1GAP, ARF1-directed GTPase-activating protein, ARF1 GAP, ARF GAP 1, ADP-ribosylation factor GTPase-activating protein 1

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Validation Data Gallery

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  WB analysis of PC-3 using 13571-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13571-1-AP (ARFGAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13571-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13571-1-AP (ARFGAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13571-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13571-1-AP (ARFGAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 13571-1-AP

  IP result of anti-ARFGAP1 (IP:13571-1-AP, 4ug; Detection:13571-1-AP 1:500) with mouse testis tissue lysate 4000ug.

• 

  IHC staining of human prostate cancer using 13571-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13571-1-AP (ARFGAP1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human prostate cancer using 13571-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13571-1-AP (ARFGAP1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 13571-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13571-1-AP (ARFGAP1 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: PC-3 cells, HeLa cells, human brain tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 2 publications below

Product Information

13571-1-AP targets ARFGAP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARFGAP1 fusion protein Ag4468
• Full Name: ADP-ribosylation factor GTPase activating protein 1
• Calculated Molecular Weight: 414 aa, 46 kDa
• Observed Molecular Weight: 46 kDa, 50 kDa
• GenBank Accession Number: BC028233
• Gene Symbol: ARFGAP1
• Gene ID (NCBI): 55738
• RRID: AB_2058621
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein coats deform flat lipid membranes into buds and capture membrane proteins to form transport vesicles. The assembly-disassembly cycle of the COPI coat on Golgi membranes is coupled to the GTP-GDP cycle of the small G protein ARF1. ARFGAP1 contributes to vesicle budding by increasing the GTPase activity of ARF1, because it promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. for ARFGAP1 may has a role in regulating the GTPase activity and neuronal toxicity of LRRK2; reciprocally, LRRK2 phosphorylates ArfGAP1 and is required for ArfGAP1 neuronal toxicity.

Publications

Species	Application	Title
human	WB	Nat Commun; BioE3 identifies specific substrates of ubiquitin E3 ligases; Authors - Orhi Barroso-Gomila
mouse,rat	WB,IF	PLoS Genet; GTPase activity and neuronal toxicity of Parkinson's disease-associated LRRK2 is regulated by ArfGAP1.; Authors - Klodjan Stafa ; KD Validated
human	WB, IF	FASEB J; ArfGAP1 acts as a GTPase-activating protein for human ADP-ribosylation factor-like 1 protein.; Authors - Hsiang-Pu Feng ; KD Validated
human,pig	WB	Vet Microbiol; ARFGAP1 binds to classical swine fever virus NS5A protein and enhances CSFV replication in PK-15 cells.; Authors - Liang Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (10-27-2021)

This antibody clearly label the Golgi localisation of endogenous ArfGAP1, and also gives a clean signal in WB.

• Applications: Western Blot, Immunofluorescence

FH

Cristian (Verified Customer) (04-17-2019)

Immunofluorescent analysis of (PFA 3%) fixed HeLa cells using 13571-1-AP (ARFGAP1 antibody) at dilution of 1:500 and Alexa Fluor 594-conjugated Affinipure Goat Anti-Rabbit IgG (H+L)

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HeLa

ARFGAP1 Antibody Immunofluorescence, validation (1:500 dilution) in HeLa (Cat no:13571-1-AP)