Tested Applications
Positive WB detected in | HepG2 cells, HeLa cells, mouse brain tissue |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
16519-1-AP targets ARFGAP2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen |
CatNo: Ag9740 Product name: Recombinant human ARFGAP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-344 aa of BC030148 Sequence: MAAEPNKTEIQTLFKRLRAVPTNKACFDCGAKNPSWASITYGVFLCIDCSGVHRSLGVHLSFIRSTELDSNWNWFQLRCMQVGGNANATAFFRQHGCTANDANTKYNSRAAQMYREKIRQLGSAALARHGTDLWIDNMSSAVPNHSPEKKDSDFFTEHTQPPAWDAPATEPSGTQQPAPSTESSGLAQPEHGPNTDLLGTSPKASLELKSSIIGKKKPAAAKKGLGAKKGLGAQKVSSQSFSEIERQAQVAEKLREQQAADAKKQAEESMVASMRLAYQELQIDRKKEEKKLQNLEGKKREQAERLGMGLVSRSSVSHSVLSEMQVIEQETPVSAKSSRSQLDL Predict reactive species |
Full Name | ADP-ribosylation factor GTPase activating protein 2 |
Calculated Molecular Weight | 521 aa, 57 kDa |
Observed Molecular Weight | 57-60 kDa |
GenBank Accession Number | BC030148 |
Gene Symbol | ARFGAP2 |
Gene ID (NCBI) | 84364 |
RRID | AB_2878270 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q8N6H7 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for ARFGAP2 antibody 16519-1-AP | Download protocol |
IF protocol for ARFGAP2 antibody 16519-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |