ARFGAP2 Polyclonal antibody

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• ARFGAP2 Polyclonal antibody (16519-1-AP)

ARFGAP2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 16519-1-AP Copied!

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ADP-ribosylation factor GTPase-activating protein 2, ARF GAP 2, ARFGAP 2, GTPase-activating protein ZNF289, IRZ

• 

  WB analysis of HepG2 using 16519-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16519-1-AP (ARFGAP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 16519-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16519-1-AP (ARFGAP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 16519-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16519-1-AP (ARFGAP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 16519-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16519-1-AP (ARFGAP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 16519-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16519-1-AP (ARFGAP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary cancer using 16519-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16519-1-AP (ARFGAP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 16519-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16519-1-AP (ARFGAP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse ovary using 16519-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 16519-1-AP (ARFGAP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat stomach using 16519-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 16519-1-AP (ARFGAP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse kidney using 16519-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16519-1-AP (ARFGAP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse ovary using 16519-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 16519-1-AP (ARFGAP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat ovary using 16519-1-AP

  Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 16519-1-AP (ARFGAP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 16519-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 16519-1-AP (ARFGAP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, mouse brain tissue
• Positive IHC detected in: rat stomach tissue, mouse kidney tissue, human ovary cancer tissue, human placenta tissue, mouse ovary tissue, rat ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16519-1-AP targets ARFGAP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9740

  Product name: Recombinant human ARFGAP2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-344 aa of BC030148

  Sequence: MAAEPNKTEIQTLFKRLRAVPTNKACFDCGAKNPSWASITYGVFLCIDCSGVHRSLGVHLSFIRSTELDSNWNWFQLRCMQVGGNANATAFFRQHGCTANDANTKYNSRAAQMYREKIRQLGSAALARHGTDLWIDNMSSAVPNHSPEKKDSDFFTEHTQPPAWDAPATEPSGTQQPAPSTESSGLAQPEHGPNTDLLGTSPKASLELKSSIIGKKKPAAAKKGLGAKKGLGAQKVSSQSFSEIERQAQVAEKLREQQAADAKKQAEESMVASMRLAYQELQIDRKKEEKKLQNLEGKKREQAERLGMGLVSRSSVSHSVLSEMQVIEQETPVSAKSSRSQLDL

• Full Name: ADP-ribosylation factor GTPase activating protein 2
• Calculated Molecular Weight: 521 aa, 57 kDa
• Observed Molecular Weight: 57-60 kDa
• GenBank Accession Number: BC030148
• Gene Symbol: ARFGAP2
• Gene ID (NCBI): 84364
• RRID: AB_2878270
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8N6H7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.