ARFGEF1 Polyclonal antibody, PBS Only

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• ARFGEF1 Polyclonal antibody, PBS Only (31684-1-PBS)

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  WB analysis using 31684-1-AP (same clone as 31684-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31684-1-AP (ARFGEF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31684-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 31684-1-AP (same clone as 31684-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31684-1-AP (ARFGEF1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31684-1-PBS in a different storage buffer formulation.

ARFGEF1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 31684-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

p200 ARF GEP1, P200, Brefeldin A inhibited GEP 1, BIG1, ARFGEP1

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Product Information

31684-1-PBS targets ARFGEF1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag35629

  Product name: Recombinant human ARFGEF1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 212-420 aa of NM_001413184

  Sequence: QALQEAKQMEKERHRQHHHLLQSPVSHHEPESPQLRYLPPQTVDHISQEHEGDLDLHTNDVDKSLQDDTEPENGSDISSAENEQTEADQATAAETLSKNEVLYDGENHDCEEKPQDIVQNIVEEMVNIVVGDMGEGTTINASADGNIGTIEDGSDSENIQANGIPGTPISVAYTPSLPDDRLSVSSNDTQESGNSSGPSPGAKFSHILQ

• Full Name: ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited)
• Calculated Molecular Weight: 208kDa
• Observed Molecular Weight: 210 kDa
• GenBank Accession Number: NM_001413184
• Gene Symbol: ARFGEF1
• Gene ID (NCBI): 10565
• RRID: AB_3670078
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9Y6D6
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ARFGEF1 (ADP ribosylation factor guanine nucleotide exchange factor 1), also known as BIG1 (Brefeldin A-inhibited guanine nucleotide exchange protein 1), is one of the ADP ribosylation factor (ARF) guanine nucleotide exchange factor (GEF) with a conserved catalytic sec7 domain.ARFGEF1 is connected with trafficking regulation through the trans-Golgi network and endosomes (PMID: 35090882).