ARHGAP1 Polyclonal antibody

ARHGAP1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11169-1-AP

Synonyms

ARHGAP1, CDC42GAP, p50 RhoGAP, p50rhoGAP, RHOGAP, RHOGAP1

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Validation Data Gallery

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  WB analysis of RAW 264.7 using 11169-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 11169-1-AP (ARHGAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 11169-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11169-1-AP (ARHGAP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 11169-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 11169-1-AP (ARHGAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human duodenum using 11169-1-AP

  human duodenum tissue were subjected to SDS PAGE followed by western blot with 11169-1-AP (ARHGAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human pancreas using 11169-1-AP

  human pancreas tissue were subjected to SDS PAGE followed by western blot with 11169-1-AP (ARHGAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas using 11169-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11169-1-AP (ARHGAP1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 11169-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11169-1-AP (ARHGAP1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 11169-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11169-1-AP (ARHGAP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 11169-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 11169-1-AP (ARHGAP1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: RAW 264.7 cells, human duodenum tissue, human heart tissue, human pancreas tissue, PC-12 cells
• Positive IHC detected in: human pancreas tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11169-1-AP targets ARHGAP1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARHGAP1 fusion protein Ag1651
• Full Name: Rho GTPase activating protein 1
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC018118
• Gene Symbol: ARHGAP1
• Gene ID (NCBI): 392
• RRID: AB_2289862
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.