ARHGAP11B Polyclonal antibody

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• ARHGAP11B Polyclonal antibody (16746-1-AP)

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  WB analysis using 16746-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16746-1-AP (ARHGAP11B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

ARHGAP11B Polyclonal Antibody for WB, ELISA

Cat No. 16746-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

FAM7B1, Inactive Rho GTPase-activating protein 11B, Rho-type GTPase-activating protein 11B

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, SK-N-SH cells, U-251 cells, mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16746-1-AP targets ARHGAP11B in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10155

  Product name: Recombinant human ARHGAP11B protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-267 aa of BC105788

  Sequence: MWDQRLVKLALLQHLRAFYGIKVKGVRGQCDRRRHETAATEIGGKIFGVPFNALPHSAVPEYGHIPSFLVDACTSLEEHIHTEGLFRKSGSVIRLKALKNKVDHGEGCLSSAPPCDIAGLLKQFFRELPEPILPADLHEALLKAQQLGTEEKNKAILLLSCLLADHTVHVLRYFFNFLRNVSLRSSENKMDSSNLAVIFAPNLLQTSEGHEKMSSNAEKKGVYQTLSWKRYQPCWVLMVSVLLHHWKALKKVNMKLLVNIREREDNV

• Full Name: Rho GTPase activating protein 11B
• Calculated Molecular Weight: 267 aa, 30 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC105788
• Gene Symbol: ARHGAP11B
• Gene ID (NCBI): 89839
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q3KRB8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARHGAP11B is a hominin-specific protein that promotes the development and evolutionary expansion of the brain neocortex(PMID: 33938018). ARHGAP11B arose from partial duplication of ARHGAP11A on the human lineage after separation from the chimpanzee lineage, but before the divergence from Neandertals. The antibody may cross-react with the high homology N-terminal domain of the rodent Arhgap11a, specifically with its short splicing variants, mimicking the human protein size.(PMID: 27957544)