ARHGAP12 Polyclonal antibody

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  IHC staining of mouse cerebellum using 17855-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 17855-1-AP (ARHGAP12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 17855-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17855-1-AP (ARHGAP12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 17855-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17855-1-AP (ARHGAP12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 17855-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17855-1-AP (ARHGAP12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ARHGAP12 Polyclonal Antibody for IHC, ELISA

Cat No. 17855-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Synonyms

Rho GTPase-activating protein 12, Rho-type GTPase-activating protein 12

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Tested Applications

• Positive IHC detected in: human kidney tissue, mouse cerebellum tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17855-1-AP targets ARHGAP12 in IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12309

  Product name: Recombinant human ARHGAP12 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 486-841 aa of BC094719

  Sequence: AVLQGSSLLFTKTQGSSTSWFGSNQSKPEFTVDLKGATIEMASKDKSSKKNVFELKTRQGTELLIQSDNDTVINDWFKVLSSTINNQAVETDEGIEEEIPDSPGIEKHDKEKEQKDPKKLRSFKVSSIDSSEQKKTKKNLKKFLTRRPTLQAVREKGYIKDQVFGSNLANLCQRENGTVPKFVKLCIEHVEEHGLDIDGIYRVSGNLAVIQKLRFAVNHDEKLDLNDSKWEDIHVITGALKMFFRELPEPLFTFNHFNDFVNAIKQEPRQRVAAVKDLIRQLPKPNQDTMQILFRHLRRVIENGEKNRMTYQSIAIVFGPTLLKPEKETGNIAVHTVYQNQIVELILLELSSIFGR

• Full Name: Rho GTPase activating protein 12
• Calculated Molecular Weight: 841 aa, 96 kDa
• GenBank Accession Number: BC094719
• Gene Symbol: ARHGAP12
• Gene ID (NCBI): 94134
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IWW6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARHGAP12 belongs to the Rho GTPase-activating protein (RhoGAP) family. RhoGAP is a key regulatory factor of the small GTPase Rho family and serves as a molecular switch that regulates various cellular processes. ARHGAP12 is involved in suppressing tumor formation by regulating cell invasion and adhesion.