ARHGAP18 Polyclonal antibody

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  WB analysis using 28409-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28409-1-AP (ARHGAP18 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 28409-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 28409-1-AP (ARHGAP18 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

ARHGAP18 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 28409-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Rho-type GTPase-activating protein 18, Rho GTPase-activating protein 18, MacGAP

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Tested Applications

• Positive WB detected in: HeLa cells, MDA-MB-453s cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28409-1-AP targets ARHGAP18 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag29144

  Product name: Recombinant human ARHGAP18 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 175-399 aa of BC107416

  Sequence: FAQQRESKETAPGGTESQSLRTNENKYQGRDDEASNLVGEEKLIPPEETPAPETDINLEVSFAEQALNQKESSKEKIQKSKGDDATLPSFRLPKDKTGTTRIGDLAPQDMKKVCHLALIELTALYDVLGIELKQQKAVKIKTKDSGLFCVPLTALLEQDQRKVPGMRIPLIFQKLISRIEERGLETEGLLRIPGAAIRIKNLCQELEAKFYEGTFNWESVKQHDA

• Full Name: Rho GTPase activating protein 18
• Calculated Molecular Weight: 663 aa, 75 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC107416
• Gene Symbol: ARHGAP18
• Gene ID (NCBI): 93663
• RRID: AB_2881136
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8N392
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.