ARHGAP23 Polyclonal antibody

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  WB analysis of A549 using 31938-1-AP

  WB result of ARHGAP23 antibody (31938-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARHGAP23 transfected A549 cells.

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  WB analysis using 31938-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31938-1-AP (ARHGAP23 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 31938-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31938-1-AP (ARHGAP23 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 31938-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31938-1-AP (ARHGAP23 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• KD/KO Validated

ARHGAP23 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 31938-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

KIAA1501, Rho GTPase Activating Protein 23, Rho GTPase-activating protein 23, Rho-type GTPase-activating protein 23

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Tested Applications

• Positive WB detected in: A549 cells, Calu-1 cells, HeLa cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31938-1-AP targets ARHGAP23 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag36340

  Product name: Recombinant human ARHGAP23 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 500-700 aa of NM_001199417

  Sequence: KVQLTPARQMNLGFGDESPEPEASGRGERLGRKVAPLATTEDSLASIPFIDEPTSPSIDLQAKHVPASAVVSSAMNSAPVLGTSPSSPTFTFTLGRHYSQDCSSIKAGRRSSYLLAITTERSKSCDDGLNTFRDEGRVLRRLPNRIPSLRMLRSFFTDGSLDSWGTSEDADAPSKRHSTSDLSDATFSDIRREGWLYYKQI

• Full Name: Rho GTPase activating protein 23
• Calculated Molecular Weight: 162kDa
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: NM_001199417
• Gene Symbol: ARHGAP23
• Gene ID (NCBI): 57636
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9P227
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARHGAP23 (Rho GTPase Activating Protein 23) belongs to the family of Rho GTPase activating proteins. The protein functions as a GTPase-activating factor by converting Rho class GTPases into their inactive GDP-bound state. ARHGAP23 is involved in the Rho GTPase signaling pathway, affecting processes such as cytoskeletal reorganization, cell migration, and cell proliferation. In non-small cell lung cancer, the expression of ARHGAP23 is associated with tumor immune infiltration and prognosis, and its role in tumor development has attracted attention. Mutations in this gene are related to a variety of diseases, including certain types of cancer and neurological disorders.