ARHGAP24 Polyclonal antibody

ARHGAP24 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, Canine

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 18834-1-AP

Synonyms

ARHGAP24, Filamin A associated RhoGAP, FILGAP, p73, p73RhoGAP, RC GAP72, RCGAP72, Rho type GTPase activating protein 24, RhoGAP of 73 kDa, Sarcoma antigen NY SAR 88

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Validation Data Gallery

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  IHC staining of human kidney using 18834-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18834-1-AP (ARHGAP24 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 18834-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18834-1-AP (ARHGAP24 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

18834-1-AP targets ARHGAP24 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat, Canine samples.

• Tested Reactivity: human, mouse, rat, Canine
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: Rho GTPase activating protein 24
• Calculated Molecular Weight: 84 kDa
• Observed Molecular Weight: 73 kDa
• GenBank Accession Number: BC098580
• Gene Symbol: ARHGAP24
• Gene ID (NCBI): 83478
• RRID: AB_10603354
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARHGAP24, also named as FILGAP, is involved in cell polarity, cell morphology and cytoskeletal organization. ARHGAP24 acts as a GTPase activator for the Rac-type GTPase by converting it to an inactive GDP-bound state. ARHGAP24 controls actin remodeling by inactivating Rac downstream of Rho leading to suppress leading edge protrusion and promotes cell retraction to achieve cellular polarity. ARHGAP24 can suppress the activity of RAC1 and CDC42 in vitro. Its overexpression induces cell rounding with partial or complete disruption of actin stress fibers and formation of membrane ruffles, lamellipodia, and filopodia. Isoform 2 is a vascular cell-specific GAP involved in modulation of angiogenesis. The antibody may recognize all the isoforms of ARHGAP24 except isoform 5.

Publications

Species	Application	Title
human	IF	Curr Biol; Cyclic Mechanical Loading Is Essential for Rac1-Mediated Elongation and Remodeling of the Embryonic Mitral Valve.; Authors - Russell A Gould
human	WB,IHC	Oncogene; Smoking-associated upregulation of CBX3 suppresses ARHGAP24 expression to activate Rac1 signaling and promote tumor progression in lung adenocarcinoma.; Authors - Xin Jin
human	IHC	Oncotarget; Global gene expression in pseudomyxoma peritonei, with parallel development of two immortalized cell lines.; Authors - Darren L Roberts
human	WB	Clin Exp Pharmacol Physiol; ARHGAP17 inhibits pathological cyclic strain-induced apoptosis in human periodontal ligament fibroblasts via Rac1/Cdc42.; Authors - Li Wang
human,mouse	WB,IF	Biochem Biophys Rep; Knockdown of ARHGAP24 reduces intimal hyperplasia through inhibiting the proliferation and phenotypic switching of smooth muscle cells possibly by inactivating both AKT and ERK1/2 signaling pathways; Authors - Wei Liu