ARHGAP27 Polyclonal antibody

ARHGAP27 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 22077-1-AP

Synonyms

SH3 domain-containing protein 20, Rho-type GTPase-activating protein 27, Rho GTPase-activating protein 27, PP905, CIN85-associated multi-domain-containing Rho GTPase-activating protein 1

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Validation Data Gallery

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  WB analysis of A549 using 22077-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 22077-1-AP (ARHGAP27 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 22077-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22077-1-AP (ARHGAP27 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 22077-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22077-1-AP (ARHGAP27 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 22077-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22077-1-AP (ARHGAP27 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 22077-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22077-1-AP (ARHGAP27 antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 22077-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 22077-1-AP (ARHGAP27 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells
• Positive IHC detected in: human breast cancer tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

22077-1-AP targets ARHGAP27 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARHGAP27 fusion protein Ag17225
• Full Name: Rho GTPase activating protein 27
• Calculated Molecular Weight: 548 aa, 62 kDa
• Observed Molecular Weight: 98 kDa
• GenBank Accession Number: BC101388
• Gene Symbol: ARHGAP27
• Gene ID (NCBI): 201176
• RRID: AB_2878985
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	iScience; Targeting ALDOA to modulate tumorigenesis and energy metabolism in retinoblastoma; Authors - Yinghao Wang