ARHGAP27 Polyclonal antibody

ARHGAP27 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 22077-1-AP

Synonyms

ARHGAP27, CAMGAP1, SH3D20



Tested Applications

Positive WB detected inA549 cells
Positive IHC detected inhuman breast cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22077-1-AP is ARHGAP27 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARHGAP27 fusion protein Ag17225
Full Name Rho GTPase activating protein 27
Calculated molecular weight 548 aa, 62 kDa
Observed molecular weight 98 kDa
GenBank accession numberBC101388
Gene symbol ARHGAP27
Gene ID (NCBI) 201176
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ARHGAP27 antibody 22077-1-APDownload protocol
IHC protocol for ARHGAP27 antibody 22077-1-APDownload protocol
IF protocol for ARHGAP27 antibody 22077-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols